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Literature summary extracted from

  • Benedetti, C.E.; Arruda, P.
    Altering the expression of the chlorophyllase gene ATHCOR1 in transgenic Arabidopsis caused changes in the chlorophyll-to-chlorophyllide ratio (2002), Plant Physiol., 128, 1255-1263.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.14 wounding induces enzyme expression from gene ATHCOR1 in leaves Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.14 expression of gene ATHCOR1 in Escherichia coli BL21 as maltose-binding protein fusion protein, cloning of the gene into an expression vector for transformation of Arabidopsis thaliana plants Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.14 additional information gene ATHCOR1: construction of transgenic Arabidopsis thaliana plants overexpressing the enzyme via infection with Agrobacterium tumefaciens and transformation, sense and antisense orientation, the sense mutation changed the chlorophyll a to chlorophyll b ratio, overview, gene AtCLH2: cloning and analysis Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.14 Triton X-100 strong inhibition at 0.2-1.0% Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.14 additional information divalent cations stimulate Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.14 chlorophyll a/b + H2O Arabidopsis thaliana the enzyme is involved in the regulation of chlorophyll a and b content phytol + chlorophyllide
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.14 Acetone strong reduction of activity at above 40% Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Arabidopsis thaliana
-
 ecotype Columbia Col-0, gene ATHCOR1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.14 recombinant maltose-binding fusion protein from Escherichia coli Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.14 chlorophyll + H2O = phytol + chlorophyllide also catalyzes chlorophyllide transfer, e.g. converts chlorophyll to methylchlorophyllide Arabidopsis thaliana
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.14 flower developing, constitutive expression of gene AtChl2 and gene ATHCOR1, expression of recmbinant enzyme in mutant transgenic plants Arabidopsis thaliana
-
3.1.1.14 flower bud AtChl2 Arabidopsis thaliana
-
3.1.1.14 leaf low content of ATHCOR1, expression of AtChl2 Arabidopsis thaliana
-
3.1.1.14 additional information not in root Arabidopsis thaliana
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.14 4°C, stable Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.14 chlorophyll a/b + H2O specific for Arabidopsis thaliana phytol + chlorophyllide
-
 ?
3.1.1.14 chlorophyll a/b + H2O the enzyme is involved in the regulation of chlorophyll a and b content Arabidopsis thaliana phytol + chlorophyllide
-
 ?
3.1.1.14 additional information no activity with 4-nitrophenyl esters of fatty acids and tributyrin Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.14 Chlase
-
 Arabidopsis thaliana
3.1.1.14 coronatine-induced protein
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.14 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 7
-
 assay at Arabidopsis thaliana