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Literature summary extracted from

  • Reverdatto, S.; Beilinson, V.; Nielsen, N.C.
    A multisubunit acetyl coenzyme A carboxylase from soybean (1999), Plant Physiol., 119, 961-978.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.2 DNA of different subunits imported into isolated chloroplast from Pisum sativum, proteins expressed in Escherichia coli accumulate in inclusion bodies Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.4.1.2 plastid
-
 Glycine max 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 600000
-
 greater than 600000 Da, gel filtration, complex from alpha and beta subunit of caroxyltransferase Glycine max
6.4.1.2 800000
-
 greater than 800000 Da, gel filtration, complex from biotin carboxylase and biotin carboxyl carrier protein Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Pisum sativum key enzyme of fatty acid biosynthesis ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Glycine max key enzyme of fatty acid biosynthesis ADP + phosphate + malonyl-CoA
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
6.4.1.2 guanidine inclusion bodies soluble in 6 M urea Glycine max
6.4.1.2 urea inclusion bodies soluble in 6 M urea Glycine max

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Glycine max
-
-
-
6.4.1.2 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Pisum sativum ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Glycine max ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- key enzyme of fatty acid biosynthesis Pisum sativum ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- key enzyme of fatty acid biosynthesis Glycine max ADP + phosphate + malonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 More multisubunit enzyme consisting of biotin carboxylase, biotin carboxyl carrier protein and the alpha and beta subunits of carboxyltransferase Pisum sativum
6.4.1.2 More multisubunit enzyme consisting of biotin carboxylase, biotin carboxyl carrier protein and the alpha and beta subunits of carboxyltransferase, protein dissociates into two complexes in presence of 0.5 M KCl, when subunit complexes of biotin carboxylase and biotin carboxyl carrier protein and alpha and beta subunits of carboxyltransferase are expressed separately the activity of full size protein can be achieved by mixing the enzyme subunit complexes Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 biotin enzyme-bound Pisum sativum
6.4.1.2 biotin enzyme-bound Glycine max