Literature summary extracted from
Chen, Y.R.; Hartman, F.C.; Lu, T.Y.; Larimer, F.W.
D-Ribulose-5-phosphate 3-epimerase: cloning and heterologous expression of the spinach gene, and purification and characterization of the recombinant enzyme (1998), Plant Physiol., 118, 199-207.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.1.3.1 |
- |
Spinacia oleracea |
General Stability
EC Number |
General Stability |
Organism |
---|
5.1.3.1 |
DL-alpha-glycerophosphate or ethanol stabilize the extremely labile recombinant enzyme, but are unable to reverse the spontaneous loss of activity |
Spinacia oleracea |
5.1.3.1 |
drastically destabilized by 2-mercaptoethanol |
Spinacia oleracea |
5.1.3.1 |
DTT destabilizes |
Spinacia oleracea |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.1.3.1 |
DL-alpha-glycerophosphate |
competitive |
Spinacia oleracea |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
5.1.3.1 |
0.22 |
- |
D-ribulose 5-phosphate |
25°C, pH 8.0 |
Spinacia oleracea |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.1.3.1 |
additional information |
no requirement for a divalent metal ion in catalysis |
Spinacia oleracea |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.1.3.1 |
25000 |
- |
8 * 25000, SDS-PAGE |
Spinacia oleracea |
5.1.3.1 |
200000 |
- |
gel filtration |
Spinacia oleracea |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.1.3.1 |
Spinacia oleracea |
Q43157 |
- |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
5.1.3.1 |
11610 |
- |
- |
Spinacia oleracea |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
5.1.3.1 |
2°C, wether in crude extracts or highly purified, the recombinant enzyme is inherently unstable |
Spinacia oleracea |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.1.3.1 |
D-Ribulose 5-phosphate |
- |
Spinacia oleracea |
D-Xylulose 5-phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.1.3.1 |
octamer |
8 * 25000, SDS-PAGE |
Spinacia oleracea |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.1.3.1 |
Ru5P epimerase |
- |
Spinacia oleracea |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
5.1.3.1 |
0.9 |
- |
DL-alpha-glycerophosphate |
25°C, pH 8.0 |
Spinacia oleracea |
|