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Literature summary extracted from

  • Blondal, T.; Hjorleifsdottir, S.H.; Fridjonsson, O.F.; Aevarsson, A.; Skirnisdottir, S.; Hermannsdottir, A.G.; Hreggvidsson, G.O.; Smith, A.V.; Kristjansson, J.K.
    Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 (2003), Nucleic Acids Res., 31, 7247-7254.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.3 RnlA gene overexpressed in Escherichia coli BL21 Rhodothermus phage RM378

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.3 NaCl
-
 Rhodothermus phage RM378

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.3 Mg2+ optimum activity at 5 mM Rhodothermus phage RM378
6.5.1.3 Mn2+ optimum activity at 1 mM Rhodothermus phage RM378

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.3 51300
-
 calculated from the putative RNA ligase 1 gene Rhodothermus phage RM378

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m Rhodothermus phage RM378
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Rhodothermus phage RM378
-
 thermophilic bacteriophage that infects the thermophilic eubacterium Rhodothermus marinus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3 recombinant enzyme Rhodothermus phage RM378

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Rhodothermus phage RM378 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 bacteriophage RNA ligase
-
 Rhodothermus phage RM378
6.5.1.3 RM378 RNA ligase
-
 Rhodothermus phage RM378
6.5.1.3 RnlA
-
 Rhodothermus phage RM378
6.5.1.3 thermostable RNA ligase 1
-
 Rhodothermus phage RM378

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.3 64
-
-
 Rhodothermus phage RM378

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.3 60 90 relatively stable at 60°C with a half-life of 10 h, activity increases after incubation, not stable at temperatures. Enzyme starts to lose activity rapidly, no activity at all after 1 h at 80°C, loses all of its activity after 5 min at 90°C Rhodothermus phage RM378

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.3 6
-
 Tris buffer Rhodothermus phage RM378
6.5.1.3 7
-
 MOPS buffer Rhodothermus phage RM378