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Literature summary extracted from

  • Inoue, T.; Irikura, D.; Okazaki, N.; Kinugasa, S.; Matsumura, H.; Uodome, N.; Yamamoto, M.; Kumasaka, T.; Miyano, M.; Kai, Y.; Urade, Y.
    Mechanism of metal activation of human hematopoietic prostaglandin D synthase (2003), Nat. Struct. Biol., 10, 291-296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.2
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.99.2 macro-seeding using a solution containing 14% polyethylene glycol 6000, 50 mM Tris-HCl, pH 8.4, 5 mM reduced glutathione, 5 mM dithiothreitol, 2.5 mM CaCl2 or MgCl2 and 1% dioxane, structure resolution at 1.8 A Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.2 D93N not activated by Ca2+ or Mg2+ Homo sapiens
5.3.99.2 D96N very high basal activity, no activation in the presence of Ca2+ or Mg2+ Homo sapiens
5.3.99.2 D97N not activated by Ca2+ or Mg2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.99.2 Ba2+ increase in activity Homo sapiens
5.3.99.2 Ca2+ half maximal effective concentration at 0.4 mM, 1.5fold increase in activity Homo sapiens
5.3.99.2 Mg2+ half maximal effective concentration at 0.05 mM, 1.5fold increase in activity, Mg2+ increases the affinity for glutathione Homo sapiens
5.3.99.2 Sr2+ increase in activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.2 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione Homo sapiens
-
 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.2 Homo sapiens O60760
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.2 recombinant PDGS Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.2 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione
-
 Homo sapiens (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.99.2 dimer crystallization Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.3.99.2 Hematopoietic prostaglandin D synthase i.e. H-PGDS Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.99.2 glutathione
-
 Homo sapiens