Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stroupe, M.E.; Leech, H.K.; Daniels, D.S.; Warren, M.J.; Getzoff, E.D.
    CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis (2003), Nat. Struct. Biol., 10, 1064-1073.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.76 recombinantly overproduced in Escherichia coli Salmonella enterica
4.99.1.4
-
 Salmonella enterica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.76 orthorhombic space group P2(1)2(1)2(1), unit cell dimensions a = 60.73 A, b = 121.49 A, c = 130.79 A Salmonella enterica
4.99.1.4 X-ray crystal structure of CysG Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.76 K270I site-directed mutagenesis Salmonella enterica
1.3.1.76 L250A site-directed mutagenesis Salmonella enterica
1.3.1.76 N385A site-directed mutagenesis Salmonella enterica
1.3.1.76 S128A site-directed mutagenesis Salmonella enterica
1.3.1.76 S128D site-directed mutagenesis Salmonella enterica
4.99.1.4 S128A mutant has higher cobalt chelatase activity than wild-type CysG with sirohydrochlorin as substrate Salmonella enterica
4.99.1.4 S128D mutant has lower cobalt chelatase activity than wild-type CysG with sirohydrochlorin as substrate Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.76 50000
-
 2 * 50000 Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+ Salmonella enterica tetrapyrrole and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ Salmonella enterica CysG, siroheme biosynthesis siroheme + 2 H+ sulfur metabolism depends on siroheme ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.76 Salmonella enterica
-
-
-
4.99.1.4 Salmonella enterica
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.3.1.76 phosphoprotein
-
 Salmonella enterica
4.99.1.4 phosphoprotein CysG, phosphorylation of Ser-128, plays a regulatory role Salmonella enterica

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.4
-
 Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.76 0.00088
-
 dehydrogenase activity, mutant S128D Salmonella enterica
1.3.1.76 0.00993
-
 dehydrogenase activity, wild-type Salmonella enterica
1.3.1.76 0.0332
-
 dehydrogenase activity, mutant S128A Salmonella enterica
4.99.1.4 0.0138
-
 S128D mutant CysG, cobalt chelation of sirohydrochlorin Salmonella enterica
4.99.1.4 0.0656
-
 wild-type CysG, cobalt chelation of sirohydrochlorin Salmonella enterica
4.99.1.4 0.243
-
 S128A mutant CysG, cobalt chelation of sirohydrochlorin Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.76 additional information large multifunctional protein that catalyzes four diverse reactions, 2 S-adensyl-L-methionine-dependent methylations, NAD+-dependent tetrapyrrole dehydrogenation and metal chelation Salmonella enterica ?
-
 ?
1.3.1.76 precorrin-2 + NAD+
-
 Salmonella enterica sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ tetrapyrrole and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III Salmonella enterica sirohydrochlorin + NADH + H+
-
 ?
4.99.1.4 sirohydrochlorin + Co2+
-
 Salmonella enterica cobalt-sirohydrochlorin + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ CysG structure, the multifunctional siroheme synthase CysG synthesizes siroheme from uroporphyrinogen III, CysG contains two structurally independent modules: a bismethyltransferase and a dual-function dehydrogenase-chelatase Salmonella enterica siroheme + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ CysG, siroheme biosynthesis Salmonella enterica siroheme + 2 H+ sulfur metabolism depends on siroheme ?

Subunits

EC Number Subunits Comment Organism
1.3.1.76 homodimer 2 * 50000 Salmonella enterica
4.99.1.4 homodimer
-
 Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
1.3.1.76 CysG
-
 Salmonella enterica
1.3.1.76 EC 2.1.1.107 multifunctional protein, first of three steps leading to formation of siroheme from uroporphyrinogen III Salmonella enterica
1.3.1.76 S-adenosyl-L-methionine(SAM)-dependent bismethyltransferase, dehydrogenase and ferrochelatase
-
 Salmonella enterica
4.99.1.4 CysG Salmonella enterica Salmonella enterica