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Literature summary extracted from
- A novel enzyme, citryl-CoA lyase, catalysing the second step of the citrate cleavage reaction in Hydrogenobacter thermophilus TK-6 (2004), Mol. Microbiol., 52, 763-770.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.3.34 | cloned, sequenced and expressed in Escherichia coli BL21(DE3) | Hydrogenobacter thermophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.34 | ATP | - |
Hydrogenobacter thermophilus |  |
| 4.1.3.34 | citrate | - |
Hydrogenobacter thermophilus | |
| 4.1.3.34 | CoA | - |
Hydrogenobacter thermophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.34 | 0.05 | - |
acetyl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
| 4.1.3.34 | 0.082 | - |
citryl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
| 4.1.3.34 | 0.13 | - |
oxaloacetate | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.34 | 30000 | - |
3 * 30000, homotrimer, SDS-PAGE | Hydrogenobacter thermophilus |
| 4.1.3.34 | 87000 | - |
gel filtration | Hydrogenobacter thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.34 | (3S)-citryl-CoA | Hydrogenobacter thermophilus | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | acetyl-CoA + oxaloacetate | - |
r | |
| 4.1.3.34 | (3S)-citryl-CoA | Hydrogenobacter thermophilus TK-6 / IAM 12695 | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | acetyl-CoA + oxaloacetate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.34 | Hydrogenobacter thermophilus | - |
- |
- |
| 4.1.3.34 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.34 | native and recombinant enzyme | Hydrogenobacter thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.34 | 1 | - |
citrate synthase activity as a side reaction | Hydrogenobacter thermophilus |
| 4.1.3.34 | 33 | - |
citrate, ATP, CoA and citryl-CoA forming enzyme CCS in the enzyme assay | Hydrogenobacter thermophilus |
| 4.1.3.34 | 280 | - |
purified citryl-CoA as substrate | Hydrogenobacter thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.34 | (3S)-citryl-CoA | - |
Hydrogenobacter thermophilus | acetyl-CoA + oxaloacetate | - |
r | |
| 4.1.3.34 | (3S)-citryl-CoA | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | Hydrogenobacter thermophilus | acetyl-CoA + oxaloacetate | - |
r | |
| 4.1.3.34 | (3S)-citryl-CoA | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + oxaloacetate | - |
r | |
| 4.1.3.34 | (3S)-citryl-CoA | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + oxaloacetate | - |
r | |
| 4.1.3.34 | additional information | shows low citrate synthase activity | Hydrogenobacter thermophilus | ? | - |
? | |
| 4.1.3.34 | additional information | shows low citrate synthase activity | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.34 | trimer | 3 * 30000, homotrimer, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.3.34 | CCL | - |
Hydrogenobacter thermophilus |
| 4.1.3.34 | citryl-CoA lyase | - |
Hydrogenobacter thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.34 | 80 | 90 | thermostable with no detectable loss of activity at 80°C for 10 min, retains 83% after 90°C for 10 min | Hydrogenobacter thermophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.34 | 570 | - |
citryl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.34 | 8 | 8.5 | - |
Hydrogenobacter thermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.34 | 7 | 9.5 | 10% of maximum activity at pH 7.0, 50% of maximum activity at pH 7.5, 90% of maximum activity at pH 9.0, 30% of maximum activity at pH 9.5 | Hydrogenobacter thermophilus |
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