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Literature summary extracted from
- Overexpression of the potential herbicide target sedoheptulose-1,7-bisphosphatase from Spinacia oleracea in transgenic tobacco (2002), Mol. Breed., 9, 53-61.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.37 | dithiothreitol | activation | Spinacia oleracea |  |
| 3.1.3.37 | sedoheptulose 1,7-bisphosphate | activation | Spinacia oleracea | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.37 | Ca2+ | activation | Spinacia oleracea | |
| 3.1.3.37 | Mg2+ | required, optimal at 15 mM | Spinacia oleracea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.37 | 35000 | - |
x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence | Spinacia oleracea |
| 3.1.3.37 | 38000 | - |
x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence | Spinacia oleracea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.37 | Spinacia oleracea | - |
overexpression in Nicotiana tabacum | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.3.37 | proteolytic modification | processing of 67 amino acids transit peptide | Spinacia oleracea |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.37 | 3.5 | - |
substrate fructose 1,6-bisphosphate, pH 8.3, 22°C | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.37 | fructose 1,6-bisphosphate + H2O | about 20-25% of the activity obtained with seduheptulose 1,7-bisphosphate | Spinacia oleracea | phosphate + ? | - |
? | |
| 3.1.3.37 | sedoheptulose 1,7-bisphosphate + H2O | - |
Spinacia oleracea | sedoheptulose 7-phosphate + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.37 | ? | x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence | Spinacia oleracea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.37 | 8.3 | - |
- |
Spinacia oleracea |
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Release 2023.1 (January 2023)
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