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Literature summary extracted from

  • Seshaiah, P.; Andrew, D.J.
    WRS-85D: A tryptophanyl-tRNA synthetase expressed to high levels in the developing Drosophila salivary gland (1999), Mol. Biol. Cell, 10, 1595-1608.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.2 the house-keeping gene maps to the cytological position 85D and is therefore termed WRS-85D, expression is dependent on the homeotic gene Sex combs reduced, i.e. Scr, ectopic expression of Scr results in expression of WRS-85D in new locations, recombinant expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp Drosophila melanogaster enzyme is required for embryonic survival, and is essential for viability of the fly AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Drosophila melanogaster Q9U4Y0 single copy gene
-
6.1.1.2 Drosophila melanogaster Q9U4Y1 single copy gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.2 recombinant His-tagged enzyme form Escherichia coli strain BL21(DE3) Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.2 embryo
-
 Drosophila melanogaster
-
6.1.1.2 larva
-
 Drosophila melanogaster
-
6.1.1.2 additional information enzyme-deficiency is lethal, accumulation pattern after treatment with interferons alpha, beta, and gamma Drosophila melanogaster
-
6.1.1.2 salivary gland embryonic, developing Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp wild-type and recombinant enzyme are specific for tRNATrp Drosophila melanogaster AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp enzyme is required for embryonic survival, and is essential for viability of the fly Drosophila melanogaster AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.2 TrpRS
-
 Drosophila melanogaster
6.1.1.2 Tryptophanyl-tRNA synthase
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.2 30
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.2 8
-
 assay at Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.2 ATP
-
 Drosophila melanogaster