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Literature summary extracted from
- Pyrrolidone carboxylpeptidase from Thermococcus litoralis (2001), Methods Enzymol., 330, 394-403.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.3 | DTT | enhances activity | Thermococcus litoralis |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.19.3 | overexpression in Escherichia coli | Thermococcus litoralis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.19.3 | crystallized from both ammonium phosphate and ammonium sulfate, structure determined at 1.7 A resolution | Thermococcus litoralis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.3 | NEM | - |
Thermococcus litoralis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 24472 | - |
4 * 24472, denaturing PAGE | Thermococcus litoralis |
| 3.4.19.3 | 96000 | - |
gel filtration | Thermococcus litoralis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.3 | Thermococcus litoralis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.3 | - |
Thermococcus litoralis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | additional information | - |
- |
Thermococcus litoralis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.3 | L-pyroglutamyl-beta-naphthylamide + H2O | - |
Thermococcus litoralis | L-pyroglutamate + beta-naphthylamine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.3 | tetramer | 4 * 24472, denaturing PAGE | Thermococcus litoralis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.19.3 | PCP | - |
Thermococcus litoralis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 70 | - |
- |
Thermococcus litoralis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 50 | 80 | 50°C: about 50% of maximal activity, 80°C: about 40% of maximal activity | Thermococcus litoralis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 70 | - |
half-life: 1 h | Thermococcus litoralis |
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