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Literature summary extracted from

  • Van Veldhoven, P.P.
    Sphingosine-1-phosphate lyase (2000), Methods Enzymol., 311, 244-254.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.27 2-vinyl sphinganine phosphate 50% inhibition at 0.0024 mM Rattus norvegicus
4.1.2.27 4-chloromercuribenzoate alkylates a cysteine residue Mammalia
4.1.2.27 Ca2+ potent inhibitor Mammalia
4.1.2.27 cyanide inhibits Mammalia
4.1.2.27 deoxypyridoxin phosphate inhibits Mammalia
4.1.2.27 iodoacetamide alkylates a cysteine residue Mammalia
4.1.2.27 N,N-dimethyldodecylamine N-oxide 50% inhibition at 0.05 mM Mammalia
4.1.2.27 N-ethylmaleimide alkylates a cysteine residue Mammalia
4.1.2.27 Semicarbazide inhibits Mammalia
4.1.2.27 sphinganine 1-phosphonate competitive inhibitor Rattus norvegicus
4.1.2.27 sphinganine phosphate 2D,3L-isomer is a competitive inhibitor, but 2L,3L-isomer does not influence the enzyme Tetrahymena pyriformis
4.1.2.27 Zn2+ potent inhibitor Mammalia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.27 0.009 0.016 sphinganine 1-phosphate
-
 Rattus norvegicus
4.1.2.27 0.11
-
 sphinganine 1-phosphate
-
 Tetrahymena pyriformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.2.27 endoplasmic reticulum catalytic site faces the cytosol Mammalia 5783
-
4.1.2.27 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.27 61000 65000 SDS-PAGE Mus musculus
4.1.2.27 61000 65000 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.27 additional information Mammalia acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.27 Homo sapiens
-
-
-
4.1.2.27 Mammalia
-
-
-
4.1.2.27 Mus musculus
-
-
-
4.1.2.27 Rattus norvegicus
-
-
-
4.1.2.27 Tetrahymena pyriformis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.2.27 Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde mechanism Mammalia
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.27 liver
-
 Rattus norvegicus
-
4.1.2.27 additional information no activity in platelets Mammalia
-

Storage Stability

EC Number Storage Stability Organism
4.1.2.27 -80°C, activity is well preserved in tissues or cells Mammalia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.27 additional information acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine Mammalia ?
-
 ?
4.1.2.27 additional information acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine Mus musculus ?
-
 ?
4.1.2.27 additional information acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine Homo sapiens ?
-
 ?
4.1.2.27 sphinganine 1-phosphate
-
 Rattus norvegicus phosphoethanolamine + palmitaldehyde
-
 ?
4.1.2.27 sphinganine 1-phosphate
-
 Tetrahymena pyriformis phosphoethanolamine + palmitaldehyde
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.27 7.4
-
 assay at Mammalia

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.27 0.005
-
 sphinganine 1-phosphonate
-
 Rattus norvegicus
4.1.2.27 0.0097
-
 sphinganine phosphate 2D,3L-isomer Tetrahymena pyriformis