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Literature summary extracted from

  • Ruiz, B.; Farres, A.; Langley, E.; Masso, F.; Sanchez, S.
    Purification and characterization of an extracellular lipase from Penicillium candidum (2001), Lipids, 36, 283-289.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Ca2+ slight activation Penicillium candidum
3.1.1.3 Nonidet P-40
-
 Penicillium candidum
3.1.1.3 nonionic detergents stimulation Penicillium candidum
3.1.1.3 Triton X-100
-
 Penicillium candidum
3.1.1.3 Tween 20
-
 Penicillium candidum
3.1.1.3 Zwittergent
-
 Penicillium candidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cu2+ about 75% inhibition at 1 mM Penicillium candidum
3.1.1.3 Fe2+ complete inhibition at 1 mM Penicillium candidum
3.1.1.3 ionic detergents
-
 Penicillium candidum
3.1.1.3 sodium cholate
-
 Penicillium candidum
3.1.1.3 Tween 80
-
 Penicillium candidum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular secretion Penicillium candidum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 29000
-
 1 * 29000, SDS-PAGE Penicillium candidum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Penicillium candidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 36.7fold, to homogeneity Penicillium candidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 13900
-
 purified enzyme, substrate 4-nitrophenyl laurate Penicillium candidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl butyrate + H2O low activity Penicillium candidum 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O
-
 Penicillium candidum 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl hexanoate + H2O low activity Penicillium candidum 4-nitrophenol + hexanoate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Penicillium candidum 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl octanoate + H2O low activity Penicillium candidum 4-nitrophenol + octanoate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Penicillium candidum 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl stearate + H2O
-
 Penicillium candidum 4-nitrophenol + stearate
-
 ?
3.1.1.3 additional information substrate specificity Penicillium candidum ?
-
 ?
3.1.1.3 tributyrin + H2O best substrate Penicillium candidum dibutyrin + butyrate
-
 ?
3.1.1.3 tridecanin + H2O
-
 Penicillium candidum didecanin + decanoate
-
 ?
3.1.1.3 tridodecanin + H2O
-
 Penicillium candidum didodecanin + dodecanoate
-
 ?
3.1.1.3 trihexanin + H2O
-
 Penicillium candidum dihexanin + hexanoate
-
 ?
3.1.1.3 trimyristin + H2O
-
 Penicillium candidum dimyristin + myristate
-
 ?
3.1.1.3 triolein + H2O
-
 Penicillium candidum diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Penicillium candidum dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O
-
 Penicillium candidum distearin + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 29000, SDS-PAGE Penicillium candidum
3.1.1.3 More the enzyme forms dimers and higher molecular weight aggregates under denaturing conditions Penicillium candidum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Penicillium candidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 35
-
-
 Penicillium candidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 25
-
 above, rapid loss of activity Penicillium candidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9
-
-
 Penicillium candidum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Penicillium candidum isoelectric focusing
-
 5.5