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Literature summary extracted from
- Enzymatic characterization of a novel phospholipase A2 from Crotalus durissus cascavella rattlesnake (Maracamboia) venom (2000), J. Protein Chem., 19, 603-607.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | crotapotin | allosteric inhibitor, 50% inhibition at 0.001 mg/ml, maximal inhibition at 0.002 mg/ml | Crotalus durissus cascavella | |
| 3.1.1.4 | Cu2+ | 10 mM | Crotalus durissus cascavella |  |
| 3.1.1.4 | heparin | allosteric inhibitor | Crotalus durissus cascavella | |
| 3.1.1.4 | Zn2+ | 10 mM | Crotalus durissus cascavella | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | Ca2+ | requirement, optimal concentration: 10 mM | Crotalus durissus cascavella | |
| 3.1.1.4 | Mg2+ | 10 mM, increases activity slightly in presence of 1 mM Ca2+ | Crotalus durissus cascavella | |
| 3.1.1.4 | Mn2+ | 10 mM, increases activity slightly in presence of 1 mM Ca2+ | Crotalus durissus cascavella | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 15000 | - |
SDS-PAGE | Crotalus durissus cascavella |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | Crotalus durissus cascavella | - |
rattlesnake | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.4 | - |
Crotalus durissus cascavella |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | venom | - |
Crotalus durissus cascavella | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | 4-nitro-3-(octanoyloxy)benzoic acid + H2O | - |
Crotalus durissus cascavella | 3-hydroxy-4-nitrobenzoate + octanoate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 35 | 40 | - |
Crotalus durissus cascavella |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 8.3 | - |
- |
Crotalus durissus cascavella |
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Release 2023.1 (January 2023)
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