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Literature summary extracted from

  • Zhang, Q.S.; Shen, L.; Wang, E.D.; Wang, Y.L.
    Biosynthesis and characterization of 4-fluorotryptophan-labeled Escherichia coli arginyl-tRNA synthetase (1999), J. Protein Chem., 18, 187-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.19 overexpression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.19 additional information biosynthetic preparation of an enzyme where Trp are replaced by 4-fluorotryptophane Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.19 0.0025
-
 tRNAArg aminoacylation reaction Escherichia coli
6.1.1.19 0.003
-
 tRNAArg aminoacylation reaction, 4-fluorotryptophan-labeled enzyme Escherichia coli
6.1.1.19 0.012
-
 L-arginine aminoacylation reaction Escherichia coli
6.1.1.19 0.0137
-
 L-arginine aminoacylation reaction, 4-fluorotryptophan-labeled enzyme Escherichia coli
6.1.1.19 0.9
-
 ATP aminoacylation reaction Escherichia coli
6.1.1.19 1.35
-
 ATP aminoacylation reaction, 4-fluorotryptophan-labeled enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.19 additional information
-
 incorporation of 4-fluorotryptophan has little effect on activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Escherichia coli AMP + diphosphate + L-arginyl-tRNAArg
-
 ?