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Literature summary extracted from

  • Schmidt, J.J.; Bostian, K.A.
    Endoproteinase activity of type A botulinum neurotoxin: substrate requirements and activation by serum albumin (1997), J. Protein Chem., 16, 19-26.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.69 bovine serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
3.4.24.69 goat serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
3.4.24.69 horse serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
3.4.24.69 Human serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
3.4.24.69 additional information stimulation specific to serum albumins, bovine gamma globulin, ovalbumin, lysozyme, ribonuclease, and gelatin have no effect Clostridium botulinum
3.4.24.69 rabbit serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
3.4.24.69 sheep serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.69 0.58
-
 SNKTRIDEANQRATKXL pH 7.3, 37°C Clostridium botulinum
3.4.24.69 0.6
-
 SNKTRIDEAAQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 0.75
-
 SNKTRIDQANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 0.75
-
 SNKTRIDEANNRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 0.82
-
 SNKTRINEANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 0.83
-
 SNKTRIDEANBRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 0.9
-
 SNKTRIDEANQRATK pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1
-
 SNKTRIDEANQRATAML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.1
-
 SNKTRIDEBNQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.2
-
 SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.3
-
 SNKTRIDEANQRABKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.6
-
 SNKTRIDEANQRATKM pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.7
-
 SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.8
-
 SNKTRIDEANQRBTKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.9
-
 SNKTRIDEANQRATKAL pH 7.3, 37°C Clostridium botulinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.69 Zn2+ zinc-dependent endoproteinase Clostridium botulinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.69 additional information Clostridium botulinum most potent toxin known ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.69 Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.69 additional information most potent toxin known Clostridium botulinum ?
-
 ?
3.4.24.69 neuronal proteinSNAP-25 + H2O
-
 Clostridium botulinum ?
-
 ?
3.4.24.69 SNKTRIDEAAQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEAAQ + RATKML
-
 ?
3.4.24.69 SNKTRIDEANBRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANB + RATKML
-
 ?
3.4.24.69 SNKTRIDEANNRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANN + RATKML
-
 ?
3.4.24.69 SNKTRIDEANQRABKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RABKML
-
 ?
3.4.24.69 SNKTRIDEANQRASKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RASKML
-
 ?
3.4.24.69 SNKTRIDEANQRATAML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATAML
-
 ?
3.4.24.69 SNKTRIDEANQRATK + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATK
-
 ?
3.4.24.69 SNKTRIDEANQRATKAL + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKAL
-
 ?
3.4.24.69 SNKTRIDEANQRATKM + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKM
-
 ?
3.4.24.69 SNKTRIDEANQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKML
-
 ?
3.4.24.69 SNKTRIDEANQRATKXL + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKXL
-
 ?
3.4.24.69 SNKTRIDEANQRBTKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RBTKML
-
 ?
3.4.24.69 SNKTRIDEBNQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEBNQ + RATKML
-
 ?
3.4.24.69 SNKTRIDQANQRATKML + H2O
-
 Clostridium botulinum ?
-
 ?
3.4.24.69 SNKTRINEAAQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRINEAAQ + RATKML
-
 ?
3.4.24.69 SNKTRINEANQRATKML + H2O
-
 Clostridium botulinum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.69 botox A
-
 Clostridium botulinum
3.4.24.69 type A botulinum neurotoxin
-
 Clostridium botulinum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.69 1
-
 SNKTRIDEANQRATK pH 7.3, 37°C Clostridium botulinum
3.4.24.69 1.8
-
 SNKTRIDEAAQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 5
-
 SNKTRINEANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 7.7
-
 SNKTRIDEANBRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 8
-
 SNKTRIDEANQRATAML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 9
-
 SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 11
-
 SNKTRIDEBNQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 19
-
 SNKTRIDEANNRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 25
-
 SNKTRIDEANQRATKAL pH 7.3, 37°C Clostridium botulinum
3.4.24.69 30
-
 SNKTRIDEANQRATKXL pH 7.3, 37°C Clostridium botulinum
3.4.24.69 35
-
 SNKTRIDEANQRABKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 39
-
 SNKTRIDEANQRBTKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 47
-
 SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 51
-
 SNKTRIDQANQRATKML pH 7.3, 37°C Clostridium botulinum
3.4.24.69 56
-
 SNKTRIDEANQRATKM pH 7.3, 37°C Clostridium botulinum