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Literature summary extracted from

  • Kambourova, M.; Kirilova, N.; Mandeva, R.; Derekova, A.
    Purification and properties of thermostable lipase from a thermophilic Bacillus stearothermophilus MC7 (2003), J. Mol. Catal. B, 22, 307-313.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 sorbitol 50%, 30 min, 70°C, pH 8.5, 10% activation Geobacillus stearothermophilus

Application

EC Number Application Comment Organism
3.1.1.3 synthesis possible industrial application of the enzyme in continuous processes due to effective immobilization on diverse materials, overview Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Co2+ 5 mM, 30 min, 70°C, pH 8.5, 65% inhibition Geobacillus stearothermophilus
3.1.1.3 Cu2+ 5 mM, 30 min, 70°C, pH 8.5, complete inhibition Geobacillus stearothermophilus
3.1.1.3 dithiothreitol 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition Geobacillus stearothermophilus
3.1.1.3 Fe2+ 5 mM, 30 min, 70°C, pH 8.5, 90% inhibition Geobacillus stearothermophilus
3.1.1.3 Hg2+ 5 mM, 30 min, 70°C, pH 8.5, 55% inhibition Geobacillus stearothermophilus
3.1.1.3 Li+ 5 mM, 30 min, 70°C, pH 8.5, 15% inhibition Geobacillus stearothermophilus
3.1.1.3 Mg2+ 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition Geobacillus stearothermophilus
3.1.1.3 Mn2+ 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition Geobacillus stearothermophilus
3.1.1.3 additional information no inhibition by iodoacetamide, K+, Na+ at 5 mM Geobacillus stearothermophilus
3.1.1.3 N-bromosuccinimide 5 mM, 30 min, 70°C, pH 8.5, 55% inhibition Geobacillus stearothermophilus
3.1.1.3 p-chloromercuribenzoate 5 mM, 30 min, 70°C, pH 8.5, 70% inhibition Geobacillus stearothermophilus
3.1.1.3 PMSF 5 mM, 30 min, 70°C, pH 8.5, complete inhibition Geobacillus stearothermophilus
3.1.1.3 Zn2+ 5 mM, 30 min, 70°C, pH 8.5, 85% inhibition Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.33
-
 4-nitrophenyl palmitate pH 8.5, 60°C Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Geobacillus stearothermophilus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ 10% stimulation at 5 mM Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 62500
-
 x * 62500, SDS-PAGE Geobacillus stearothermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Acetone 50%, 30 min, 70°C, pH 8.5, loss of 60% activity Geobacillus stearothermophilus
3.1.1.3 Butanol 50%, 30 min, 70°C, pH 8.5, complete loss of activity Geobacillus stearothermophilus
3.1.1.3 chloroform 50%, 30 min, 70°C, pH 8.5, complete loss of activity Geobacillus stearothermophilus
3.1.1.3 diethyl ether 50%, 30 min, 70°C, pH 8.5, complete loss of activity Geobacillus stearothermophilus
3.1.1.3 Ethanol 50%, 30 min, 70°C, pH 8.5, loss of 70% activity Geobacillus stearothermophilus
3.1.1.3 Glycerol 50%, 30 min, 70°C, pH 8.5, loss of 60% activity Geobacillus stearothermophilus
3.1.1.3 isopropanol 50%, 30 min, 70°C, pH 8.5, loss of 60% activity Geobacillus stearothermophilus
3.1.1.3 Methanol 50%, 30 min, 70°C, pH 8.5, loss of 20% activity Geobacillus stearothermophilus
3.1.1.3 n-hexane 50%, 30 min, 70°C, pH 8.5, loss of 85% activity Geobacillus stearothermophilus
3.1.1.3 sorbitol 50%, 30 min, 70°C, pH 8.5, 10% activation Geobacillus stearothermophilus
3.1.1.3 toluol 50%, 30 min, 70°C, pH 8.5, complete loss of activity Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus stearothermophilus
-
 thermostable lipase
-
3.1.1.3 Geobacillus stearothermophilus MC 7
-
 thermostable lipase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 19.25fold, from culture supernatant Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 11.9
-
 purified enzyme, substrate 4-nitrophenyl palmitate Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Geobacillus stearothermophilus MC 7 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Geobacillus stearothermophilus 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Geobacillus stearothermophilus MC 7 4-nitrophenol + palmitate
-
 ?
3.1.1.3 DL-alpha-palmitin + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.1.1.3 DL-alpha-stearin + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.1.1.3 additional information the enzyme also possesses esterase activity Geobacillus stearothermophilus ?
-
 ?
3.1.1.3 additional information the enzyme also possesses esterase activity Geobacillus stearothermophilus MC 7 ?
-
 ?
3.1.1.3 olive oil + H2O emulsion Geobacillus stearothermophilus ?
-
 ?
3.1.1.3 triacetin + H2O
-
 Geobacillus stearothermophilus diacetin + acetate
-
 ?
3.1.1.3 tributyrin + H2O best substrate Geobacillus stearothermophilus dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O best substrate Geobacillus stearothermophilus MC 7 dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O
-
 Geobacillus stearothermophilus dicaprin + capriate
-
 ?
3.1.1.3 trilaurin + H2O
-
 Geobacillus stearothermophilus dilaurin + laurate
-
 ?
3.1.1.3 triolein + H2O
-
 Geobacillus stearothermophilus diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Geobacillus stearothermophilus MC 7 diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Geobacillus stearothermophilus dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O
-
 Geobacillus stearothermophilus distearin + stearate
-
 ?
3.1.1.3 Tween 80 + H2O i.e. poly(oxyethylene) sorbitan monooleate Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 62500, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 75 80
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 55 90
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 70
-
 purified enzyme: half-life is 30 min, crude enzyme: half-life is 3 h Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7.5 9
-
 Geobacillus stearothermophilus