BRENDA - Enzyme Database

Insights into the catalytic mechanism of cofactor-independent phosphoglycerate mutase from X-ray crystallography, simulated dynamics and molecular modeling

Rigden, D.J.; Lamani, E.; Mello, L.V.; Littlejohn, J.E.; Jedrzejas, M.J.; J. Mol. Biol. 328, 909-920 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Geobacillus stearothermophilus
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.12
crystals of wild-type and S62A mutant at 1.4 A resolution
Geobacillus stearothermophilus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.12
S62A
no phosphoglycerate mutase activity
Geobacillus stearothermophilus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Geobacillus stearothermophilus
-
3-phospho-D-glycerate
-
Geobacillus stearothermophilus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Geobacillus stearothermophilus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
recombinant phosphoglycerate mutase
Geobacillus stearothermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
652915
Geobacillus stearothermophilus
3-phospho-D-glycerate
-
652915
Geobacillus stearothermophilus
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Geobacillus stearothermophilus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Geobacillus stearothermophilus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Geobacillus stearothermophilus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.12
crystals of wild-type and S62A mutant at 1.4 A resolution
Geobacillus stearothermophilus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.12
S62A
no phosphoglycerate mutase activity
Geobacillus stearothermophilus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Geobacillus stearothermophilus
-
3-phospho-D-glycerate
-
Geobacillus stearothermophilus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
recombinant phosphoglycerate mutase
Geobacillus stearothermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
652915
Geobacillus stearothermophilus
3-phospho-D-glycerate
-
652915
Geobacillus stearothermophilus
r