BRENDA - Enzyme Database

Polyanionic inhibitors of phosphoglycerate mutase: combined structural and biochemical analysis

Rigden, D.J.; Walter, R.A.; Phillips, S.E.; Fothergill-Gilmore, L.A.; J. Mol. Biol. 289, 691-699 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
5.4.2.11
2,3-bisphosphoglycerate
-
Schizosaccharomyces pombe
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.11
crystallization of PGAM complexed with inositol hexakisphosphate and benzene hexacarboxylate, crystal structures at 2.5-2.8 A resolution
Saccharomyces cerevisiae
General Stability
EC Number
General Stability
Organism
5.4.2.11
little effect of the protease thermolysin on enzyme activity
Schizosaccharomyces pombe
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.11
benzene hexacarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene hexacarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,2,3-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,2,3-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,2,4,5-tetracarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,2,4,5-tetracarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,2,4-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,2,4-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,3,5-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,3,5-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
Inositol hexakisphosphate
-
Saccharomyces cerevisiae
5.4.2.11
Inositol hexakisphosphate
-
Schizosaccharomyces pombe
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.56
-
3-phosphoglycerate
-
Saccharomyces cerevisiae
5.4.2.11
0.58
-
3-phosphoglycerate
-
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae
r
5.4.2.11
2-phospho-D-glycerate
Schizosaccharomyces pombe
-
3-phospho-D-glycerate
-
Schizosaccharomyces pombe
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.11
Saccharomyces cerevisiae
P00950
-
-
5.4.2.11
Schizosaccharomyces pombe
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.11
-
Saccharomyces cerevisiae
5.4.2.11
-
Schizosaccharomyces pombe
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
652799
Saccharomyces cerevisiae
3-phospho-D-glycerate
-
652799
Saccharomyces cerevisiae
r
5.4.2.11
2-phospho-D-glycerate
-
652799
Schizosaccharomyces pombe
3-phospho-D-glycerate
-
652799
Schizosaccharomyces pombe
r
5.4.2.11
3-Phosphoglycerate
-
652799
Schizosaccharomyces pombe
2-Phosphoglycerate
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.11
82
-
3-phospho-D-glycerate
-
Schizosaccharomyces pombe
5.4.2.11
530
-
3-phosphoglycerate
-
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.4.2.11
0.004
-
Inositol hexakisphosphate
-
Saccharomyces cerevisiae
5.4.2.11
0.006
-
benzene hexacarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.013
-
benzene-1,2,4,5-tetracarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.021
-
benzene-1,2,4-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.022
-
benzene hexacarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.023
-
benzene-1,2,3-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.024
-
benzene-1,3,5-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.035
-
Inositol hexakisphosphate
-
Schizosaccharomyces pombe
5.4.2.11
0.053
-
benzene-1,3,5-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.055
-
benzene-1,2,4,5-tetracarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.062
-
benzene-1,2,4-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.067
-
benzene-1,2,3-tricarboxylate
-
Schizosaccharomyces pombe
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
5.4.2.11
2,3-bisphosphoglycerate
-
Schizosaccharomyces pombe
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.11
crystallization of PGAM complexed with inositol hexakisphosphate and benzene hexacarboxylate, crystal structures at 2.5-2.8 A resolution
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
5.4.2.11
little effect of the protease thermolysin on enzyme activity
Schizosaccharomyces pombe
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.11
benzene hexacarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene hexacarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,2,3-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,2,3-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,2,4,5-tetracarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,2,4,5-tetracarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,2,4-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,2,4-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
benzene-1,3,5-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
benzene-1,3,5-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
Inositol hexakisphosphate
-
Saccharomyces cerevisiae
5.4.2.11
Inositol hexakisphosphate
-
Schizosaccharomyces pombe
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.4.2.11
0.004
-
Inositol hexakisphosphate
-
Saccharomyces cerevisiae
5.4.2.11
0.006
-
benzene hexacarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.013
-
benzene-1,2,4,5-tetracarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.021
-
benzene-1,2,4-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.022
-
benzene hexacarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.023
-
benzene-1,2,3-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.024
-
benzene-1,3,5-tricarboxylate
-
Saccharomyces cerevisiae
5.4.2.11
0.035
-
Inositol hexakisphosphate
-
Schizosaccharomyces pombe
5.4.2.11
0.053
-
benzene-1,3,5-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.055
-
benzene-1,2,4,5-tetracarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.062
-
benzene-1,2,4-tricarboxylate
-
Schizosaccharomyces pombe
5.4.2.11
0.067
-
benzene-1,2,3-tricarboxylate
-
Schizosaccharomyces pombe
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.56
-
3-phosphoglycerate
-
Saccharomyces cerevisiae
5.4.2.11
0.58
-
3-phosphoglycerate
-
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae
r
5.4.2.11
2-phospho-D-glycerate
Schizosaccharomyces pombe
-
3-phospho-D-glycerate
-
Schizosaccharomyces pombe
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.11
-
Saccharomyces cerevisiae
5.4.2.11
-
Schizosaccharomyces pombe
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
652799
Saccharomyces cerevisiae
3-phospho-D-glycerate
-
652799
Saccharomyces cerevisiae
r
5.4.2.11
2-phospho-D-glycerate
-
652799
Schizosaccharomyces pombe
3-phospho-D-glycerate
-
652799
Schizosaccharomyces pombe
r
5.4.2.11
3-Phosphoglycerate
-
652799
Schizosaccharomyces pombe
2-Phosphoglycerate
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.11
82
-
3-phospho-D-glycerate
-
Schizosaccharomyces pombe
5.4.2.11
530
-
3-phosphoglycerate
-
Saccharomyces cerevisiae