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Literature summary extracted from

  • Merz, A.; Knochel, T.; Jansonius, J.N.; Kirschner, K.
    The hyperthermostable indoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridges (1999), J. Mol. Biol., 288, 753-763.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.48 D184A no alteration of native structure, very little difference in Km, turnover rate compared to wild type, but less thermal stability Thermotoga maritima
4.1.1.48 R241A no alteration of native structure, very little difference in Km, turnover rate compared to wild type, but less thermal stability Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.48 0.000006
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C Thermotoga maritima
4.1.1.48 0.000014
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 45°C Thermotoga maritima
4.1.1.48 0.000053
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 60°C Thermotoga maritima
4.1.1.48 0.000123
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 80°C Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate Thermotoga maritima
-
 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Thermotoga maritima 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.48 73
-
 335 min, half-life for irreversible thermal inactivation Thermotoga maritima
4.1.1.48 83.5
-
 110 min, half-life for irreversible thermal inactivation Thermotoga maritima
4.1.1.48 85.5
-
 14.4 min, half-life for irreversible thermal inactivation, mutant R241A Thermotoga maritima
4.1.1.48 85.5
-
 36.1 min, half-life for irreversible thermal inactivation, mutant D184A Thermotoga maritima
4.1.1.48 85.5
-
 42.3 min, half-life for irreversible thermal inactivation Thermotoga maritima
4.1.1.48 90
-
 11 min, half-life for irreversible thermal inactivation Thermotoga maritima