BRENDA - Enzyme Database

Sulphate ions observed in the 2.12 A structure of a new crystal form of S. cerevisiae phosphoglycerate mutase provide insights into understanding the catalytic mechanism

Rigden, D.J.; Walter, R.A.; Phillips, S.E.; Fothergill-Gilmore, L.A.; J. Mol. Biol. 286, 1507-1517 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.11
overexpression in Saccharomyces cerevisae
Saccharomyces cerevisiae
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.11
crystal structure at 2.12 a resolution
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.11
A60S
69% of wild-type activity
Saccharomyces cerevisiae
5.4.2.11
E86Q
1% of wild-type activity
Saccharomyces cerevisiae
5.4.2.11
S11A
4% of wild-type activity
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.43
-
3-phospho-D-glycerate
A60S mutant
Saccharomyces cerevisiae
5.4.2.11
0.47
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae
5.4.2.11
0.71
-
3-phospho-D-glycerate
S11A mutant
Saccharomyces cerevisiae
5.4.2.11
1.1
-
3-phospho-D-glycerate
E86Q mutant
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.11
Saccharomyces cerevisiae
P00950
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
652790
Saccharomyces cerevisiae
3-phospho-D-glycerate
-
652790
Saccharomyces cerevisiae
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.11
4.7
-
3-phospho-D-glycerate
E86Q mutant
Saccharomyces cerevisiae
5.4.2.11
20
-
3-phospho-D-glycerate
S11A mutant
Saccharomyces cerevisiae
5.4.2.11
370
-
3-phospho-D-glycerate
A60S mutant
Saccharomyces cerevisiae
5.4.2.11
530
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.11
overexpression in Saccharomyces cerevisae
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.11
crystal structure at 2.12 a resolution
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.11
A60S
69% of wild-type activity
Saccharomyces cerevisiae
5.4.2.11
E86Q
1% of wild-type activity
Saccharomyces cerevisiae
5.4.2.11
S11A
4% of wild-type activity
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.43
-
3-phospho-D-glycerate
A60S mutant
Saccharomyces cerevisiae
5.4.2.11
0.47
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae
5.4.2.11
0.71
-
3-phospho-D-glycerate
S11A mutant
Saccharomyces cerevisiae
5.4.2.11
1.1
-
3-phospho-D-glycerate
E86Q mutant
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
652790
Saccharomyces cerevisiae
3-phospho-D-glycerate
-
652790
Saccharomyces cerevisiae
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.11
4.7
-
3-phospho-D-glycerate
E86Q mutant
Saccharomyces cerevisiae
5.4.2.11
20
-
3-phospho-D-glycerate
S11A mutant
Saccharomyces cerevisiae
5.4.2.11
370
-
3-phospho-D-glycerate
A60S mutant
Saccharomyces cerevisiae
5.4.2.11
530
-
3-phospho-D-glycerate
-
Saccharomyces cerevisiae