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Literature summary extracted from

  • Plater, A.R.; Zgiby, S.M.; Thomson, G.J.; Qamar, S.; Wharton, C.W.; Berry, A.
    Conserved residues in the mechanism of the E. coli Class II FBP-aldolase (1999), J. Mol. Biol., 285, 843-855.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.13 D109A very low catalytic activity Escherichia coli
4.1.2.13 D144A increased Km Escherichia coli
4.1.2.13 D288A increased Km Escherichia coli
4.1.2.13 D290A increased Km Escherichia coli
4.1.2.13 D329A increased Km Escherichia coli
4.1.2.13 N286A increased Km, decreased kcat Escherichia coli
4.1.2.13 N286D increased Km, severely decreased kcat Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.13 dihydroxyacetone phosphate
-
 Escherichia coli
4.1.2.13 glyceraldehyde 3-phosphate non-competitive inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.18
-
 D-fructose 1,6-bisphosphate 30°C, D109A mutant Escherichia coli
4.1.2.13 0.19
-
 D-fructose 1,6-bisphosphate 30°C, wild-type enzyme Escherichia coli
4.1.2.13 0.37
-
 D-fructose 1,6-bisphosphate 30°C, N286D mutant Escherichia coli
4.1.2.13 0.77
-
 D-fructose 1,6-bisphosphate 30°C, D290A mutant Escherichia coli
4.1.2.13 0.92
-
 D-fructose 1,6-bisphosphate 30°C, D329A mutant Escherichia coli
4.1.2.13 0.94
-
 D-fructose 1,6-bisphosphate 30°C, D144A mutant Escherichia coli
4.1.2.13 1
-
 D-fructose 1,6-bisphosphate 30°C, D288A mutant Escherichia coli
4.1.2.13 1.07
-
 D-fructose 1,6-bisphosphate 30°C, N286A mutant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate Escherichia coli
-
 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Escherichia coli P0AB71
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.2.13 D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate mechanism of class II aldolase Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Escherichia coli glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.13 0.001
-
 D-fructose 1,6-bisphosphate 30°C, N286D mutant Escherichia coli
4.1.2.13 0.0028
-
 D-fructose 1,6-bisphosphate 30°C, D109A mutant Escherichia coli
4.1.2.13 0.19
-
 D-fructose 1,6-bisphosphate 30°C, N286A mutant Escherichia coli
4.1.2.13 2
-
 D-fructose 1,6-bisphosphate 30°C, D144A mutant Escherichia coli
4.1.2.13 2.2
-
 D-fructose 1,6-bisphosphate 30°C, D288A mutant Escherichia coli
4.1.2.13 7
-
 D-fructose 1,6-bisphosphate 30°C, D290A mutant Escherichia coli
4.1.2.13 8.2
-
 D-fructose 1,6-bisphosphate 30°C, wild-type enzyme Escherichia coli
4.1.2.13 12.3
-
 D-fructose 1,6-bisphosphate 30°C, D329A mutant Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.13 0.13
-
 dihydroxyacetone phosphate 30°C, wild-type enzyme Escherichia coli
4.1.2.13 1.7
-
 dihydroxyacetone phosphate 30°C, N268A mutant Escherichia coli