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Literature summary extracted from

  • Waterworth, W.M.; Jiang, Q.; West, C.E.; Nikaido, M.; Bray, C.M.
    Characterization of Arabidopsis photolyase enzymes and analysis of their role in protection from ultraviolet-B radiation (2002), J. Exp. Bot., 53, 1005-1015.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.3 6xHis-tagged protein expressed in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.3 additional information CPD but not 6-4PP enzyme activity exhibits a lagperiod in photoreactivation after 3 days dark exposure Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.3 60000
-
 6xHis-tagged protein, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.3 additional information Arabidopsis thaliana light-dependent repair of UV-induced damage products in DNA by direct reversal of base damage rather than via excision repair pathways ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.3 Arabidopsis thaliana Q9SB00
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.3
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.99.3 flower
-
 Arabidopsis thaliana
-
4.1.99.3 leaf
-
 Arabidopsis thaliana
-
4.1.99.3 additional information no activity in root Arabidopsis thaliana
-
4.1.99.3 silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.3 cyclobutadipyrimidine in DNA two photolyases specific for photoreactivation of either cyclobutane pyrimidine dimers or pyrimidine (6-4)pyrimidones Arabidopsis thaliana 2 pyrimidine residues in DNA
-
 ?
4.1.99.3 additional information light-dependent repair of UV-induced damage products in DNA by direct reversal of base damage rather than via excision repair pathways Arabidopsis thaliana ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.3 15
-
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.99.3 15 37 decrease in photoreactivation capacity with increasing temperature Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.3 FAD
-
 Arabidopsis thaliana
4.1.99.3 pterin contains not only reduced FAD but also a reduced pterin, or a cofactor with similar properties, as a chromophore Arabidopsis thaliana