BRENDA - Enzyme Database show

Inhibition of hyaluronidases and chondroitinases by fatty acids

Suzuki, K.; Terasaki, Y.; Uyeda, M.; J. Enzyme Inhib. Med. Chem. 17, 183-186 (2002)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.2.1
(13Z)-docos-13-enoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
(13Z)-docos-13-enoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
arachidic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
arachidic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
behenic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
behenic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
capric acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
capric acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosadienoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosadienoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosanoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosanoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosapentaenoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosapentaenoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosatetraenoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosatetraenoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosatrienoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosatrienoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
elaidic acid
-
Streptococcus dysgalactiae
4.2.2.1
elaidic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
lauric acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
lauric acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
linoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
linoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
linolenic acid
-
Streptococcus dysgalactiae
4.2.2.1
linolenic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
methyl (9Z)-octadecenoate
-
Streptococcus dysgalactiae
4.2.2.1
methyl (9Z)-octadecenoate
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
myristic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
myristic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
myristoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
myristoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
nervonic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
nervonic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
oleic acid
-
Streptococcus dysgalactiae
4.2.2.1
oleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
palmitic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
palmitic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
palmitoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
palmitoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
petroselinic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
petroselinic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
ricinoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
ricinoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
stearic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
stearic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
vaccenic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
vaccenic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.5
(13Z)-docos-13-enoic acid
-
Paenarthrobacter aurescens
4.2.2.5
(13Z)-docos-13-enoic acid
-
Pedobacter heparinus
4.2.2.5
arachidic acid
-
Paenarthrobacter aurescens
4.2.2.5
arachidic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
behenic acid
-
Paenarthrobacter aurescens
4.2.2.5
behenic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
capric acid
weak inhibition
Paenarthrobacter aurescens
4.2.2.5
capric acid
weak inhibition
Pedobacter heparinus
4.2.2.5
eicosadienoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosadienoic acid
-
Pedobacter heparinus
4.2.2.5
eicosanoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosanoic acid
-
Pedobacter heparinus
4.2.2.5
eicosapentaenoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosapentaenoic acid
-
Pedobacter heparinus
4.2.2.5
eicosatetraenoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosatetraenoic acid
-
Pedobacter heparinus
4.2.2.5
eicosatrienoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosatrienoic acid
-
Pedobacter heparinus
4.2.2.5
elaidic acid
-
Paenarthrobacter aurescens
4.2.2.5
elaidic acid
-
Pedobacter heparinus
4.2.2.5
lauric acid
weak inhibition
Paenarthrobacter aurescens
4.2.2.5
lauric acid
weak inhibition
Pedobacter heparinus
4.2.2.5
linoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
linoleic acid
-
Pedobacter heparinus
4.2.2.5
linolenic acid
-
Paenarthrobacter aurescens
4.2.2.5
linolenic acid
-
Pedobacter heparinus
4.2.2.5
methyl (9Z)-octadecenoate
weak inhibition
Paenarthrobacter aurescens
4.2.2.5
methyl (9Z)-octadecenoate
weak inhibition
Pedobacter heparinus
4.2.2.5
additional information
degree of inhibition by fatty acids depends on the chain length and the number of unsaturated bonds as well as the stereochemistry, overview
Paenarthrobacter aurescens
4.2.2.5
additional information
degree of inhibition by fatty acids depends on the chain length and the number of unsaturated bonds as well as the stereochemistry, overview
Pedobacter heparinus
4.2.2.5
myristic acid
-
Paenarthrobacter aurescens
4.2.2.5
myristic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
myristoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
myristoleic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
nervonic acid
-
Paenarthrobacter aurescens
4.2.2.5
nervonic acid
-
Pedobacter heparinus
4.2.2.5
oleic acid
-
Paenarthrobacter aurescens
4.2.2.5
oleic acid
-
Pedobacter heparinus
4.2.2.5
palmitic acid
-
Paenarthrobacter aurescens
4.2.2.5
palmitic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
palmitoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
palmitoleic acid
-
Pedobacter heparinus
4.2.2.5
petroselinic acid
-
Paenarthrobacter aurescens
4.2.2.5
petroselinic acid
-
Pedobacter heparinus
4.2.2.5
ricinoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
ricinoleic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
stearic acid
-
Paenarthrobacter aurescens
4.2.2.5
stearic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
vaccenic acid
-
Paenarthrobacter aurescens
4.2.2.5
vaccenic acid
-
Pedobacter heparinus
4.2.2.19
(13Z)-docos-13-enoic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
arachidic acid
-
Pedobacter heparinus
4.2.2.19
behenic acid
-
Pedobacter heparinus
4.2.2.19
capric acid
-
Pedobacter heparinus
4.2.2.19
eicosadienoic acid
-
Pedobacter heparinus
4.2.2.19
eicosanoic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
eicosapentaenoic acid
-
Pedobacter heparinus
4.2.2.19
eicosatetraenoic acid
-
Pedobacter heparinus
4.2.2.19
eicosatrienoic acid
; nonconmpetitive
Pedobacter heparinus
4.2.2.19
elaidic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
lauric acid
-
Pedobacter heparinus
4.2.2.19
linoleic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
linolenic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
methyl (9Z)-octadecenoate
-
Pedobacter heparinus
4.2.2.19
additional information
degree of inhibition by fatty acids depends on the chain length and the number of unsaturated bonds as well as the stereochemistry, overview
Pedobacter heparinus
4.2.2.19
myristic acid
-
Pedobacter heparinus
4.2.2.19
myristoleic acid
-
Pedobacter heparinus
4.2.2.19
nervonic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
oleic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
palmitic acid
-
Pedobacter heparinus
4.2.2.19
palmitoleic acid
-
Pedobacter heparinus
4.2.2.19
petroselinic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
ricinoleic acid
-
Pedobacter heparinus
4.2.2.19
stearic acid
-
Pedobacter heparinus
4.2.2.19
vaccenic acid
strong inhibition
Pedobacter heparinus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.2.5
0.008
-
chondroitin sulfate C
pH 6.0, 37°C
Pedobacter heparinus
4.2.2.5
0.0104
-
chondroitin sulfate C
pH 6.0, 37°C
Paenarthrobacter aurescens
4.2.2.19
0.0163
-
dermatan sulfate
pH 8.0, 37°C
Pedobacter heparinus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.2.1
Streptococcus dysgalactiae
-
-
-
4.2.2.1
Streptomyces hyalurolytics
-
-
-
4.2.2.5
Paenarthrobacter aurescens
-
-
-
4.2.2.5
Pedobacter heparinus
-
-
-
4.2.2.19
Pedobacter heparinus
Q46079
-
-
Reaction
EC Number
Reaction
Commentary
Organism
4.2.2.1
Cleaves hyaluronate chains at a beta-D-GalNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
also acts on chondroitin, the product is more systematically known as 3-(4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-2-acetamido-2-deoxy-D-glucose
Streptomyces hyalurolytics
4.2.2.1
Cleaves hyaluronate chains at a beta-D-GalNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
also acts on chondroitin, the product is more systematically known as 3-(4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-2-acetamido-2-deoxy-D-glucose
Streptococcus dysgalactiae
4.2.2.5
Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups
acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate
Paenarthrobacter aurescens
4.2.2.5
Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups
acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate
Pedobacter heparinus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.2.1
hyaluronic acid
from pig skin
652640
Streptococcus dysgalactiae
?
-
-
-
?
4.2.2.1
hyaluronic acid
from pig skin
652640
Streptomyces hyalurolytics
3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
-
-
?
4.2.2.5
chondroitin sulfate C
i.e. chondroitin 6-sulfate
652640
Pedobacter heparinus
?
-
652640
Pedobacter heparinus
?
4.2.2.5
chondroitin sulfate C
i.e. chondroitin 6-sulfate
652640
Paenarthrobacter aurescens
?
-
652640
Paenarthrobacter aurescens
?
4.2.2.19
dermatan sulfate
-
652640
Pedobacter heparinus
?
-
652640
Pedobacter heparinus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.2.1
37
-
assay at
Streptococcus dysgalactiae
4.2.2.1
60
-
assay at
Streptomyces hyalurolytics
4.2.2.5
37
-
assay at
Paenarthrobacter aurescens
4.2.2.5
37
-
assay at
Pedobacter heparinus
4.2.2.19
30
-
assay at
Pedobacter heparinus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.2.1
6.2
-
phosphate buffer, assay at
Streptococcus dysgalactiae
4.2.2.1
6.2
-
phosphate buffer, assay at
Streptomyces hyalurolytics
4.2.2.5
6
-
assay at
Paenarthrobacter aurescens
4.2.2.5
7.3
-
assay at
Pedobacter heparinus
4.2.2.19
8
-
assay at, Tris-HCl
Pedobacter heparinus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.2.1
additional information
-
additional information
-
Streptococcus dysgalactiae
4.2.2.1
additional information
-
additional information
-
Streptomyces hyalurolytics
4.2.2.5
0.0047
-
nervonic acid
pH 6.0, 37°C
Paenarthrobacter aurescens
4.2.2.5
0.0211
-
eicosatrienoic acid
pH 6.0, 37°C
Pedobacter heparinus
4.2.2.19
additional information
-
additional information
-
Pedobacter heparinus
4.2.2.19
0.037
-
eicosatrienoic acid
pH 8.0, 37°C
Pedobacter heparinus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.2.1
(13Z)-docos-13-enoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
(13Z)-docos-13-enoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
arachidic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
arachidic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
behenic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
behenic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
capric acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
capric acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosadienoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosadienoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosanoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosanoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosapentaenoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosapentaenoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosatetraenoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosatetraenoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
eicosatrienoic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
eicosatrienoic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
elaidic acid
-
Streptococcus dysgalactiae
4.2.2.1
elaidic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
lauric acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
lauric acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
linoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
linoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
linolenic acid
-
Streptococcus dysgalactiae
4.2.2.1
linolenic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
methyl (9Z)-octadecenoate
-
Streptococcus dysgalactiae
4.2.2.1
methyl (9Z)-octadecenoate
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
myristic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
myristic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
myristoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
myristoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
nervonic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
nervonic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
oleic acid
-
Streptococcus dysgalactiae
4.2.2.1
oleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
palmitic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
palmitic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
palmitoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
palmitoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
petroselinic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
petroselinic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
ricinoleic acid
-
Streptococcus dysgalactiae
4.2.2.1
ricinoleic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
stearic acid
weak inhibition
Streptococcus dysgalactiae
4.2.2.1
stearic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.1
vaccenic acid
strong inhibition
Streptococcus dysgalactiae
4.2.2.1
vaccenic acid
weak inhibition
Streptomyces hyalurolytics
4.2.2.5
(13Z)-docos-13-enoic acid
-
Paenarthrobacter aurescens
4.2.2.5
(13Z)-docos-13-enoic acid
-
Pedobacter heparinus
4.2.2.5
arachidic acid
-
Paenarthrobacter aurescens
4.2.2.5
arachidic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
behenic acid
-
Paenarthrobacter aurescens
4.2.2.5
behenic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
capric acid
weak inhibition
Paenarthrobacter aurescens
4.2.2.5
capric acid
weak inhibition
Pedobacter heparinus
4.2.2.5
eicosadienoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosadienoic acid
-
Pedobacter heparinus
4.2.2.5
eicosanoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosanoic acid
-
Pedobacter heparinus
4.2.2.5
eicosapentaenoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosapentaenoic acid
-
Pedobacter heparinus
4.2.2.5
eicosatetraenoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosatetraenoic acid
-
Pedobacter heparinus
4.2.2.5
eicosatrienoic acid
-
Paenarthrobacter aurescens
4.2.2.5
eicosatrienoic acid
-
Pedobacter heparinus
4.2.2.5
elaidic acid
-
Paenarthrobacter aurescens
4.2.2.5
elaidic acid
-
Pedobacter heparinus
4.2.2.5
lauric acid
weak inhibition
Paenarthrobacter aurescens
4.2.2.5
lauric acid
weak inhibition
Pedobacter heparinus
4.2.2.5
linoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
linoleic acid
-
Pedobacter heparinus
4.2.2.5
linolenic acid
-
Paenarthrobacter aurescens
4.2.2.5
linolenic acid
-
Pedobacter heparinus
4.2.2.5
methyl (9Z)-octadecenoate
weak inhibition
Paenarthrobacter aurescens
4.2.2.5
methyl (9Z)-octadecenoate
weak inhibition
Pedobacter heparinus
4.2.2.5
additional information
degree of inhibition by fatty acids depends on the chain length and the number of unsaturated bonds as well as the stereochemistry, overview
Paenarthrobacter aurescens
4.2.2.5
additional information
degree of inhibition by fatty acids depends on the chain length and the number of unsaturated bonds as well as the stereochemistry, overview
Pedobacter heparinus
4.2.2.5
myristic acid
-
Paenarthrobacter aurescens
4.2.2.5
myristic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
myristoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
myristoleic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
nervonic acid
-
Paenarthrobacter aurescens
4.2.2.5
nervonic acid
-
Pedobacter heparinus
4.2.2.5
oleic acid
-
Paenarthrobacter aurescens
4.2.2.5
oleic acid
-
Pedobacter heparinus
4.2.2.5
palmitic acid
-
Paenarthrobacter aurescens
4.2.2.5
palmitic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
palmitoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
palmitoleic acid
-
Pedobacter heparinus
4.2.2.5
petroselinic acid
-
Paenarthrobacter aurescens
4.2.2.5
petroselinic acid
-
Pedobacter heparinus
4.2.2.5
ricinoleic acid
-
Paenarthrobacter aurescens
4.2.2.5
ricinoleic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
stearic acid
-
Paenarthrobacter aurescens
4.2.2.5
stearic acid
weak inhibition
Pedobacter heparinus
4.2.2.5
vaccenic acid
-
Paenarthrobacter aurescens
4.2.2.5
vaccenic acid
-
Pedobacter heparinus
4.2.2.19
(13Z)-docos-13-enoic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
arachidic acid
-
Pedobacter heparinus
4.2.2.19
behenic acid
-
Pedobacter heparinus
4.2.2.19
capric acid
-
Pedobacter heparinus
4.2.2.19
eicosadienoic acid
-
Pedobacter heparinus
4.2.2.19
eicosanoic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
eicosapentaenoic acid
-
Pedobacter heparinus
4.2.2.19
eicosatetraenoic acid
-
Pedobacter heparinus
4.2.2.19
eicosatrienoic acid
nonconmpetitive
Pedobacter heparinus
4.2.2.19
eicosatrienoic acid
-
Pedobacter heparinus
4.2.2.19
elaidic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
lauric acid
-
Pedobacter heparinus
4.2.2.19
linoleic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
linolenic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
methyl (9Z)-octadecenoate
-
Pedobacter heparinus
4.2.2.19
additional information
degree of inhibition by fatty acids depends on the chain length and the number of unsaturated bonds as well as the stereochemistry, overview
Pedobacter heparinus
4.2.2.19
myristic acid
-
Pedobacter heparinus
4.2.2.19
myristoleic acid
-
Pedobacter heparinus
4.2.2.19
nervonic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
oleic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
palmitic acid
-
Pedobacter heparinus
4.2.2.19
palmitoleic acid
-
Pedobacter heparinus
4.2.2.19
petroselinic acid
strong inhibition
Pedobacter heparinus
4.2.2.19
ricinoleic acid
-
Pedobacter heparinus
4.2.2.19
stearic acid
-
Pedobacter heparinus
4.2.2.19
vaccenic acid
strong inhibition
Pedobacter heparinus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.2.1
additional information
-
additional information
-
Streptococcus dysgalactiae
4.2.2.1
additional information
-
additional information
-
Streptomyces hyalurolytics
4.2.2.5
0.0047
-
nervonic acid
pH 6.0, 37°C
Paenarthrobacter aurescens
4.2.2.5
0.0211
-
eicosatrienoic acid
pH 6.0, 37°C
Pedobacter heparinus
4.2.2.19
additional information
-
additional information
-
Pedobacter heparinus
4.2.2.19
0.037
-
eicosatrienoic acid
pH 8.0, 37°C
Pedobacter heparinus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.2.5
0.008
-
chondroitin sulfate C
pH 6.0, 37°C
Pedobacter heparinus
4.2.2.5
0.0104
-
chondroitin sulfate C
pH 6.0, 37°C
Paenarthrobacter aurescens
4.2.2.19
0.0163
-
dermatan sulfate
pH 8.0, 37°C
Pedobacter heparinus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.2.1
hyaluronic acid
from pig skin
652640
Streptococcus dysgalactiae
?
-
-
-
?
4.2.2.1
hyaluronic acid
from pig skin
652640
Streptomyces hyalurolytics
3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
-
-
?
4.2.2.5
chondroitin sulfate C
i.e. chondroitin 6-sulfate
652640
Pedobacter heparinus
?
-
652640
Pedobacter heparinus
?
4.2.2.5
chondroitin sulfate C
i.e. chondroitin 6-sulfate
652640
Paenarthrobacter aurescens
?
-
652640
Paenarthrobacter aurescens
?
4.2.2.19
dermatan sulfate
-
652640
Pedobacter heparinus
?
-
652640
Pedobacter heparinus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.2.1
37
-
assay at
Streptococcus dysgalactiae
4.2.2.1
60
-
assay at
Streptomyces hyalurolytics
4.2.2.5
37
-
assay at
Paenarthrobacter aurescens
4.2.2.5
37
-
assay at
Pedobacter heparinus
4.2.2.19
30
-
assay at
Pedobacter heparinus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.2.1
6.2
-
phosphate buffer, assay at
Streptococcus dysgalactiae
4.2.2.1
6.2
-
phosphate buffer, assay at
Streptomyces hyalurolytics
4.2.2.5
6
-
assay at
Paenarthrobacter aurescens
4.2.2.5
7.3
-
assay at
Pedobacter heparinus
4.2.2.19
8
-
assay at, Tris-HCl
Pedobacter heparinus