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Literature summary extracted from

  • Ogasahara, K.; Ishida, M.; Yutani, K.
    Stimulated interaction between and subunits of tryptophan synthase from hyperthermophile enhances its thermal stability (2003), J. Biol. Chem., 278, 8922-8928.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 genes trpA encoding the alpha-subunit and trpB encoding the beta-subunit are coexpressed or expressed individually in Escherichia coli strain JM109 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 additional information
-
 additional information thermodynamic parameters of subunit association, kinetics Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.20 27500
-
 alpha-subunit monomer, ultracentrifugation sedimentation equilibrium Pyrococcus furiosus
4.2.1.20 42500
-
 2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE Pyrococcus furiosus
4.2.1.20 42500
-
 2 * 42500, recombinant beta-subunit, SDS-PAGE Pyrococcus furiosus
4.2.1.20 84000 88000 beta-subunit dimer, ultracentrifugation sedimentation equilibrium Pyrococcus furiosus
4.2.1.20 140000
-
 enzyme complex alpha2beta2, ultracentrifugation sedimentation equilibrium Pyrococcus furiosus
4.2.1.20 275000
-
 2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 recombinant alpha-subunit, beta-subunit, and enzyme complex expressed in Escherichia coli Pyrococcus furiosus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Pyrococcus furiosus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Pyrococcus furiosus D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Pyrococcus furiosus L-tryptophan + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 dimer 2 * 42500, recombinant beta-subunit, SDS-PAGE Pyrococcus furiosus
4.2.1.20 monomer 1 * 27500, recombinant alpha-subunit, SDS-PAGE Pyrococcus furiosus
4.2.1.20 More high association constant for the hyperthermophile enzyme subunits, pH-dependence of the molecular weights Pyrococcus furiosus
4.2.1.20 tetramer 2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.20 additional information
-
 inactivation temperature of the alpha-subunit is enhanced by the formation of the enzyme complex alpha2beta2 by 17°C Pyrococcus furiosus