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Literature summary extracted from

  • Brufatto, N.; Nesheim, M.E.
    Analysis of the kinetics of prothrombin activation and evidence that two equilibrating forms of prothrombinase are involved in the process (2003), J. Biol. Chem., 278, 6755-6764.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.6 additional information
-
 additional information kinetics Homo sapiens
3.4.21.6 0.00036
-
 recombinant prothrombin R155A/R284A/R271A mutant rMZ-II pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 0.00039
-
 recombinant prothrombin R155A/R284A/R320A mutant rP2-II pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 0.00041
-
 prothrombin pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 0.00045
-
 recombinant prothrombin R155A/R284A/R271A mutant rMZ-II pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.0005
-
 recombinant prothrombin R155A/R284A/R320A mutant rP2-II pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.00058
-
 fragment 1.2:prethrombin-2 pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 0.00065
-
 meizothrombin pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 0.00068
-
 prothrombin pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.00078
-
 fragment 1.2:prethrombin-2 pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.00095
-
 meizothrombin pH 7.4, 22°C, in absence of factor Va Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.6 Ca2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.6 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.6 selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.6 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.6 fragment 1.2:prethrombin-2 + H2O intermediate of prothrombin processing to thrombin, cleavage site is Arg320 Homo sapiens thrombin
-
 ?
3.4.21.6 meizothrombin + H2O intermediate of prothrombin processing to thrombin, cleavage site is Arg271 Homo sapiens thrombin
-
 ?
3.4.21.6 prothrombin + H2O wild-type II, cleavage sites are Arg271 and Arg320 Homo sapiens thrombin + ?
-
 ?
3.4.21.6 recombinant prothrombin R155A/R284A/R271A mutant rMZ-II + H2O cleavage site is Arg320 Homo sapiens meizothrombin reaction intermediate in the reaction with wild-type prothrombin ?
3.4.21.6 recombinant prothrombin R155A/R284A/R271A/S525C mutant rMZ-II-F + H2O labeled with fluorescein at Cys525, cleavage site is Arg320 Homo sapiens meizothrombin reaction intermediate in the reaction with wild-type prothrombin ?
3.4.21.6 recombinant prothrombin R155A/R284A/R320A mutant rP2-II + H2O cleavage site is Arg271 Homo sapiens fragment 1.2:prethrombin-2 reaction intermediate in the reaction with wild-type prothrombin ?
3.4.21.6 recombinant prothrombin S525C mutant + H2O labeled with fluorescein at Cys525, cleavage sites are Arg271 and Arg320 Homo sapiens thrombin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.6 More the enzyme belongs to the peptidase family S1, i.e. trypsin family Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.6 22
-
 assay at, room temperature Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.6 additional information
-
 meizothrombin pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.0045
-
 prothrombin pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.0057
-
 fragment 1.2:prethrombin-2 pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.0068
-
 recombinant prothrombin R155A/R284A/R271A mutant rMZ-II pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 0.324
-
 recombinant prothrombin R155A/R284A/R320A mutant rP2-II pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 6.39
-
 meizothrombin pH 7.4, 22°C, in absence of factor Va Homo sapiens
3.4.21.6 108
-
 prothrombin pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 110
-
 recombinant prothrombin R155A/R284A/R271A mutant rMZ-II pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 114
-
 fragment 1.2:prethrombin-2 pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 114
-
 recombinant prothrombin R155A/R284A/R320A mutant rP2-II pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens
3.4.21.6 150
-
 meizothrombin pH 7.4, 22°C, in presence of 20 mM factor Va Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.6 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.6 Factor Va required Homo sapiens