BRENDA - Enzyme Database

Biochemical and molecular characterization of two phosphatidic acid-selective phospholipase A1s, mPA-PLA1alpha and mPA-PLA1beta

Hiramatsu, T.; Sonoda, H.; Takanezawa, Y.; Morikawa, R.; Ishida, M.; Kasahara, K.; Sanai, Y.; Taguchi, R.; Aoki, J.; Arai, H.; J. Biol. Chem. 278, 49438-49447 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.32
expression HeLa, CHO-K1 and Sf9 cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
Sodium vanadate
0.01 mM, 50% inhibition
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
52991
-
x * 52991, deduced from nucleotide sequence
Homo sapiens
3.1.1.32
55000
-
x * 55000, SDS-PAGE, immunoblot
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.32
phosphatidic acid + H2O
Homo sapiens
-
2-acyl-lysophosphatidic acid + a carboxylate
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Homo sapiens
Q6XZB0
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
testis
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidic acid + H2O
-
652486
Homo sapiens
2-acyl-lysophosphatidic acid + a carboxylate
-
652486
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 52991, deduced from nucleotide sequence; x * 55000, SDS-PAGE, immunoblot
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
expression HeLa, CHO-K1 and Sf9 cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
Sodium vanadate
0.01 mM, 50% inhibition
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
52991
-
x * 52991, deduced from nucleotide sequence
Homo sapiens
3.1.1.32
55000
-
x * 55000, SDS-PAGE, immunoblot
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.32
phosphatidic acid + H2O
Homo sapiens
-
2-acyl-lysophosphatidic acid + a carboxylate
-
Homo sapiens
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
testis
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidic acid + H2O
-
652486
Homo sapiens
2-acyl-lysophosphatidic acid + a carboxylate
-
652486
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 52991, deduced from nucleotide sequence; x * 55000, SDS-PAGE, immunoblot
Homo sapiens