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Literature summary extracted from

  • Villa, J.P.; Bertenshaw, G.P.; Bond, J.S.
    Critical Amino Acids in the Active Site of Meprin Metalloproteinases for Substrate and Peptide Bond Specificity (2003), J. Biol. Chem., 278, 42545-42550.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.18 Trypsin
-
 Mus musculus
3.4.24.63 Trypsin
-
 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.18 expressed in human embryonic kidney 293 cells ATCC 1573 CRL Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.18 F161R site-directed mutagenesis Mus musculus
3.4.24.18 P228K site-directed mutagenesis Mus musculus
3.4.24.18 Y199K site-directed mutagenesis Mus musculus
3.4.24.63 K185Y site-directed mutagenesis Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.18 0.11
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant Y199K Mus musculus
3.4.24.18 0.23
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant F161R Mus musculus
3.4.24.18 0.29
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant P228K Mus musculus
3.4.24.18 0.44
-
 gastrin 17 pH 7.5, 37°C, mutant Y199K Mus musculus
3.4.24.18 0.49
-
 cholecystokinin 8-sulfate pH 7.5, 37°C Mus musculus
3.4.24.63 0.00104
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl Mus musculus
3.4.24.63 0.0185
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl Mus musculus
3.4.24.63 0.02
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl, mutant K185Y Mus musculus
3.4.24.63 0.0296
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl, mutant K185Y Mus musculus
3.4.24.63 0.211
-
 cholecystokinin 8 sulfate pH 7.5, 37°C Mus musculus
3.4.24.63 0.22
-
 cholecystokinin 8 sulfate pH 7.5, 37°C, mutant K185Y Mus musculus
3.4.24.63 0.282
-
 alpha-MSH pH 7.5, 37°C, mutant K185Y Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.18 Zn2+ zinc-metalloendopeptidase Mus musculus
3.4.24.63 Zn2+ zinc-metalloendopeptidase Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.18 Mus musculus
-
 mouse
-
3.4.24.63 Mus musculus
-
 mouse
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.18
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.18 azocasein + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 cholecystokinin 8-sulfate + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 gastrin 17 + H2O mutant Y199K Mus musculus ?
-
 ?
3.4.24.18 additional information orcokinin and gastrin 17 are no substrates Mus musculus ?
-
 ?
3.4.24.63 alpha-MSH + H2O
-
 Mus musculus ?
-
 ?
3.4.24.63 azocasein + H2O
-
 Mus musculus ?
-
 ?
3.4.24.63 cholecystokinin 8 sulfate + H2O
-
 Mus musculus ?
-
 ?
3.4.24.63 gastrin 17 + H2O
-
 Mus musculus ?
-
 ?
3.4.24.63 additional information neurotensin is no substrate Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.18 meprin metalloproteinase
-
 Mus musculus
3.4.24.18 mouse meprin alpha
-
 Mus musculus
3.4.24.63 meprin metalloproteinase
-
 Mus musculus
3.4.24.63 mouse meprin beta
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.18 3.8
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant P228K Mus musculus
3.4.24.18 8.6
-
 cholecystokinin 8-sulfate pH 7.5, 37°C Mus musculus
3.4.24.18 10.8
-
 gastrin 17 pH 7.5, 37°C, mutant Y199K Mus musculus
3.4.24.18 13.4
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant F161R Mus musculus
3.4.24.18 14.2
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant Y199K Mus musculus
3.4.24.63 10.5
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl, mutant K185Y Mus musculus
3.4.24.63 11
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl Mus musculus
3.4.24.63 11.1
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl Mus musculus
3.4.24.63 13.9
-
 cholecystokinin 8 sulfate pH 7.5, 37°C Mus musculus
3.4.24.63 15
-
 alpha-MSH pH 7.5, 37°C, mutant K185Y Mus musculus
3.4.24.63 15
-
 cholecystokinin 8 sulfate pH 7.5, 37°C, mutant K185Y Mus musculus
3.4.24.63 17.8
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl, mutant K185Y Mus musculus