Literature summary extracted from

Yun, Y.S.; Lee, T.H.; Nam, G.H.; Jang, D.S.; Shin, S.; Oh, B.H.; Choi, K.Y.
Origin of the different pH activity profile in two homologous ketosteroid isomerases (2003), J. Biol. Chem., 278, 28229-28236.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
5.3.3.1	crystals of mutant enzyme F116W are grown by hanging-drop method	Comamonas testosteroni

Protein Variants

EC Number	Protein Variants	Comment	Organism
5.3.3.1	F116W	the turnover-number for 5-androstene-3,17-dione is lowered 4.42fold, the KM-value is 3.1fold lower than the Km-value of the wild-type enzyme	Comamonas testosteroni

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5.3.3.1	0.0486	-	5-androstene-3,17-dione	25°C, pH 7.0, mutant enzyme F116W	Comamonas testosteroni
5.3.3.1	0.1528	-	5-androstene-3,17-dione	25°C, pH 7.0, wild-type enzyme	Comamonas testosteroni

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.3.3.1	Comamonas testosteroni	P00947	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5.3.3.1	5(10)-estrene-3,17-dione	nonsticky substrate	Comamonas testosteroni	estr-4-en-3,17-dione	-	?
5.3.3.1	5-Androstene-3,17-dione	-	Comamonas testosteroni	4-Androstene-3,17-dione	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
5.3.3.1	TI-WT	-	Comamonas testosteroni

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.3.3.1	7155	-	5-androstene-3,17-dione	25°C, pH 7.0, mutant enzyme F116W	Comamonas testosteroni
5.3.3.1	7160	-	5-androstene-3,17-dione	25°C, pH 7.0, mutant enzyme F116W	Comamonas testosteroni
5.3.3.1	31680	-	5-androstene-3,17-dione	25°C, pH 7.0, wild-type enzyme	Comamonas testosteroni
5.3.3.1	31700	-	5-androstene-3,17-dione	25°C, pH 7.0, wild-type enzyme	Comamonas testosteroni

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)