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Literature summary extracted from

  • Marchesini, N.; Luberto, C.; Hannun, Y.A.
    Biochemical properties of mammalian neutral sphingomyelinase 2 and its role in sphingolipid metabolism (2003), J. Biol. Chem., 278, 13775-13783.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 cardiolipin 0.1 mM, approx. 40fold activation Mus musculus
3.1.4.12 phosphatidylserine 0.1-0.15 mM, approx. 40fold activation Mus musculus
3.1.4.12 Triton X-100 required for activity, maximal activation at 0.1% Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expression of nSMase2 in Saccharomyces cerevisiae and MCF7 breast cancer cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 GW4869 0.015 mM, 40% inhibition, 0.045 mM, 70% inhibition Mus musculus
3.1.4.12 Mn2+ slight inhibition above 2 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.027
-
 sphingomyelin 37°C, pH 7.4, recombinant nSMase2 in yeast microsomes Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mg2+ required for activity, maximal activation at 5 mM Mus musculus
3.1.4.12 Mn2+ can replace Mg2+, maximal activation at 1-2 mM, slight inhibition above Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Mus musculus
-
 N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Mus musculus Q9JJY3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Mus musculus N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 neutral sphingomyelinase
-
 Mus musculus
3.1.4.12 nSMase2
-
 Mus musculus