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Literature summary extracted from

  • Sakuraba, H.; Tsuge, H.; Shimoya, I.; Kawakami, R.; Goda, S.; Kawarabayasi, Y.; Katunuma, N.; Ago, H.; Miyano, M.; Ohshima, T.
    The first crystal structure of archaeal aldolase. Unique tetrameric structure of 2-deoxy-D-ribose-5-phosphate aldolase from the hyperthermophilic archaea Aeropyrum pernix (2003), J. Biol. Chem., 278, 10799-10806.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.4 expressen in Escherichia coli Aeropyrum pernix

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.4 hanging drop vapor diffusion method Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.4 0.057
-
 2-deoxy-D-ribose 5-phosphate 50°C, pH 6.5 Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.4 24000
-
 4 * 24000, SDS-PAGE Aeropyrum pernix
4.1.2.4 93000
-
 gel-filtration Aeropyrum pernix

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.2.4 Ethanol stable up to 40% for 30 min at 50°C Aeropyrum pernix
4.1.2.4 Methanol stable up to 50% for 30 min at 50°C Aeropyrum pernix
4.1.2.4 N,N-dimethylformamide stable up to 50% for 30 min at 50°C Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.4 Aeropyrum pernix Q9Y948 K1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.4
-
 Aeropyrum pernix

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.4 4.5
-
 50°C Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.4 2-deoxy-D-ribose 5-phosphate
-
 Aeropyrum pernix D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.4 tetramer 4 * 24000, SDS-PAGE Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.4 50
-
 assay at Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.4 80
-
 no loss of activity after 60 min Aeropyrum pernix
4.1.2.4 100
-
 no loss of activity after 10 min Aeropyrum pernix