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Literature summary extracted from

  • Jones, P.R.; Manabe, T.; Awazuhara, M.; Saito, K.
    A new member of plant CS-lyases. A cystine lyase from Arabidopsis thaliana (2003), J. Biol. Chem., 278, 10291-10296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13 expression in Escherichia coli Arabidopsis thaliana
4.4.1.13 expression in Escherichia coli Brassica oleracea
4.4.1.35 expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 2.32
-
 L-cystine
-
 Arabidopsis thaliana
4.4.1.35 2.32
-
 L-cystine pH 7.6, 22°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 47200
-
 x * 47200, calculation from nucleotide sequence Brassica oleracea
4.4.1.35 43000
-
-
 Brassica oleracea
4.4.1.35 47200
-
-
 Brassica oleracea

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Arabidopsis thaliana
-
-
-
4.4.1.13 Brassica oleracea Q84UD0
-
-
4.4.1.35 Arabidopsis thaliana Q9SUR6
-
-
4.4.1.35 Brassica oleracea Q84UD0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Arabidopsis thaliana
4.4.1.13
-
 Brassica oleracea
4.4.1.35
-
 Brassica oleracea
4.4.1.35
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cystine + H2O
-
 Arabidopsis thaliana L-thiocysteine + pyruvate + NH3
-
 ?
4.4.1.13 L-cystine + H2O
-
 Brassica oleracea L-thiocysteine + pyruvate + NH3
-
 ?
4.4.1.35 L-cystine + H2O
-
 Arabidopsis thaliana L-thiocysteine + pyruvate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 ? x * 47200, calculation from nucleotide sequence Brassica oleracea
4.4.1.35 dimer 2 * 43000, SDS-PAGE, two bands of two closely related isoforms. 2 * 47200, calculated Brassica oleracea

Synonyms

EC Number Synonyms Comment Organism
4.4.1.35 BOCL3
-
 Brassica oleracea
4.4.1.35 CORI3
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.4.1.13 Brassica oleracea calculation from nucleotide sequence
-
 8.3
4.4.1.35 Brassica oleracea calculated
-
 8.3