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Literature summary extracted from

  • Sriskanda, V.; Shuman, S.
    Conserved residues in domain Ia are required for the reaction of Escherichia coli DNA ligase with NAD+ (2002), J. Biol. Chem., 277, 9695-9700.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.5.1.2 medicine potential target for antibiotics Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expressed in Escherichia coli BL21 (DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 D32A traces of nick joining activity Escherichia coli
6.5.1.2 D32E traces of nick joining activity Escherichia coli
6.5.1.2 D32N 9% of nick joining activity, strongly reduced self-adenylation Escherichia coli
6.5.1.2 D36A traces of nick joining activity Escherichia coli
6.5.1.2 D36E 4% of nick joining activity, strongly reduced self-adenylation Escherichia coli
6.5.1.2 D36N 12% of nick joining activity, strongly reduced self-adenylation Escherichia coli
6.5.1.2 DELTA1-38 nick joining activity abolished, no self adenylation, active with pre-adenylated nick Escherichia coli
6.5.1.2 DELTA1-78 nick joining activity abolished, no self adenylation, active with pre-adenylated nick Escherichia coli
6.5.1.2 E10A 90% of nick joining activity Escherichia coli
6.5.1.2 H23A 10% of nick joining activity Escherichia coli
6.5.1.2 H23Y 88% of nick joining activity Escherichia coli
6.5.1.2 Y22A traces of nick joining activity, strongly reduced self-adenylation Escherichia coli
6.5.1.2 Y22S 9% of nick joining activity Escherichia coli
6.5.1.2 Y35A 2% of nick joining activity, strongly reduced self-adenylation Escherichia coli
6.5.1.2 Y35S 23% of nick joining activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Escherichia coli DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.2 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+ required Escherichia coli