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Literature summary extracted from

  • Gorrell, A.; Wang, W.; Underbakke, E.; Hou, Z.; Honzatko, R.B.; Fromm, H.J.
    Determinants of L-L-aspartate and IMP recognition in Escherichia coli adenylosuccinate synthetase (2002), J. Biol. Chem., 277, 8817-8821.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.4
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.4 T128A site-directed mutagenesis Escherichia coli
6.3.4.4 T129A site-directed mutagenesis Escherichia coli
6.3.4.4 T300A site-directed mutagenesis Escherichia coli
6.3.4.4 T301A site-directed mutagenesis Escherichia coli
6.3.4.4 V273A site-directed mutagenesis Escherichia coli
6.3.4.4 V273N site-directed mutagenesis Escherichia coli
6.3.4.4 V273T site-directed mutagenesis Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.4 fumarate
-
 Escherichia coli
6.3.4.4 Maleate
-
 Escherichia coli
6.3.4.4 succinate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.4 0.017
-
 IMP pH 7.7, 25°C, mutant V273A Escherichia coli
6.3.4.4 0.019
-
 IMP pH 7.7, 25°C, mutant T300A Escherichia coli
6.3.4.4 0.021
-
 GTP pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 0.024
-
 IMP pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 0.026
-
 GTP pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 0.028
-
 IMP pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 0.031
-
 GTP pH 7.7, 25°C, mutant V273A Escherichia coli
6.3.4.4 0.033
-
 IMP pH 7.7, 25°C, mutant T300V Escherichia coli
6.3.4.4 0.043
-
 IMP pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 0.045
-
 GTP pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 0.054
-
 GTP pH 7.7, 25°C, mutant T128A Escherichia coli
6.3.4.4 0.056
-
 GTP pH 7.7, 25°C, mutant T300V Escherichia coli
6.3.4.4 0.057
-
 GTP pH 7.7, 25°C, mutant T301A, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.066
-
 IMP pH 7.7, 25°C, wild-type, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.074
-
 GTP pH 7.7, 25°C, mutant V273T, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.077
-
 IMP pH 7.7, 25°C, mutant V273T, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.113
-
 IMP pH 7.7, 25°C, mutant V273N, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.14
-
 GTP pH 7.7, 25°C, mutant V273A, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.14
-
 IMP pH 7.7, 25°C, mutant V273A, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.17
-
 L-aspartate pH 7.7, 25°C, mutant T128A Escherichia coli
6.3.4.4 0.17
-
 GTP pH 7.7, 25°C, mutant T300A Escherichia coli
6.3.4.4 0.18
-
 GTP pH 7.7, 25°C, mutant V273N, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.19
-
 GTP pH 7.7, 25°C, wild-type, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.23
-
 L-aspartate pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 0.24
-
 L-aspartate pH 7.7, 25°C, mutant T129A Escherichia coli
6.3.4.4 0.31
-
 IMP pH 7.7, 25°C, mutant T128A Escherichia coli
6.3.4.4 0.32
-
 IMP pH 7.7, 25°C, mutant T301A, hydroxylamine as substrate Escherichia coli
6.3.4.4 0.82
-
 L-aspartate pH 7.7, 25°C, mutant T300V Escherichia coli
6.3.4.4 0.89
-
 IMP pH 7.7, 25°C, mutant T129A Escherichia coli
6.3.4.4 1.7
-
 GTP pH 7.7, 25°C, mutant T129A Escherichia coli
6.3.4.4 3.4
-
 L-aspartate pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 6.2
-
 L-aspartate pH 7.7, 25°C, mutant T300A Escherichia coli
6.3.4.4 7
-
 L-aspartate pH 7.7, 25°C, mutant V273A Escherichia coli
6.3.4.4 9
-
 L-aspartate pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 91
-
 hydroxylamine pH 7.7, 25°C, mutant T301A Escherichia coli
6.3.4.4 110
-
 hydroxylamine pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 140
-
 hydroxylamine pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 230
-
 hydroxylamine pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 255
-
 hydroxylamine pH 7.7, 25°C, mutant V273A Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate Escherichia coli governs the first committed step in de novo biosynthesis of AMP plays a significant role in de novo purine nucleotide biosynthesis, the purine nucleotide cycle, and/or salvage pathway for nucleotides GDP + phosphate + adenylosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Escherichia coli P0A7D4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4 wild-type and mutant enzymes Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + hydroxylamine
-
 Escherichia coli GDP + phosphate + ?
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate governs the first committed step in de novo biosynthesis of AMP plays a significant role in de novo purine nucleotide biosynthesis, the purine nucleotide cycle, and/or salvage pathway for nucleotides Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.4 homodimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.4.4 Adenylosuccinate synthetase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.4 0.05
-
 GTP pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 0.08
-
 hydroxylamine pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 0.21
-
 hydroxylamine pH 7.7, 25°C, mutant V273A Escherichia coli
6.3.4.4 0.24
-
 hydroxylamine pH 7.7, 25°C, mutant T301A Escherichia coli
6.3.4.4 0.29
-
 hydroxylamine pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 0.42
-
 GTP pH 7.7, 25°C, mutant T129A Escherichia coli
6.3.4.4 0.46
-
 GTP pH 7.7, 25°C, mutant T128A Escherichia coli
6.3.4.4 0.5
-
 hydroxylamine pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 0.64
-
 GTP pH 7.7, 25°C, mutant T300A Escherichia coli
6.3.4.4 1
-
 GTP pH 7.7, 25°C, mutant V273A Escherichia coli
6.3.4.4 1
-
 GTP pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 1.99
-
 GTP pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 2.94
-
 GTP pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 3.3
-
 GTP pH 7.7, 25°C, mutant T300V Escherichia coli
6.3.4.4 6.08
-
 GTP pH 7.7, 25°C, mutant T300A Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.4 0.04
-
 fumarate pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 0.078
-
 Maleate pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 0.08
-
 succinate pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 0.18
-
 fumarate pH 7.7, 25°C, mutant V273A Escherichia coli
6.3.4.4 0.21
-
 Maleate pH 7.7, 25°C, mutant V273N Escherichia coli
6.3.4.4 0.64
-
 fumarate pH 7.7, 25°C, mutant V273T Escherichia coli
6.3.4.4 0.89
-
 succinate pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 1
-
 Maleate pH 7.7, 25°C, mutant V273A Escherichia coli
6.3.4.4 3.1
-
 Maleate pH 7.7, 25°C, wild-type Escherichia coli