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Literature summary extracted from
- Mutational analysis of duck delta 2 crystallin and the structure of an inactive mutant with bound substrate provide insight into the enzymatic mechanism of argininosuccinate lyase (2002), J. Biol. Chem., 277, 4166-4175.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.2.1 | expression in Escherichia coli | Anas platyrhynchos |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.3.2.1 | S283A mutant, hanging-drop vapor diffusion at room temperatur, 9 mg/ml protein in 10 mM Tris-HCl, pH 7.5 and 1 mM EDTA is preincubated with 75 mM argininosuccinate, subsequently 0.005 ml drops of the solution are mixed with an equal ammount of precipitating solution consisting of 12% polyethylene glycol 2000 MME, 300 mM MgCl2, 100 mM HEPES, pH 7.4, crystals diffract to 1.96 A resolution | Anas platyrhynchos |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.3.2.1 | soluble | - |
Anas platyrhynchos | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.2.1 | (Nomega-L-arginino)succinate | Anas platyrhynchos | - |
fumarate + L-arginine | - |
r |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.2.1 | Anas platyrhynchos | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.2.1 | recombinant His-tagged ASL | Anas platyrhynchos |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.3.2.1 | eye | lens | Anas platyrhynchos | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.2.1 | (Nomega-L-arginino)succinate | - |
Anas platyrhynchos | fumarate + L-arginine | - |
? | |
| 4.3.2.1 | (Nomega-L-arginino)succinate | - |
Anas platyrhynchos | fumarate + L-arginine | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.2.1 | delta2 crystallin | - |
Anas platyrhynchos |
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Release 2023.1 (January 2023)
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