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Literature summary extracted from

  • Furusato, M.; Sueyoshi, N.; Mitsutake, S.; Sakaguchi, K.; Kita, K.; Okino, N.; Ichinose, S.; Omori, A.; Ito, M.
    Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8 (2002), J. Biol. Chem., 277, 17300-17307.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.69 taurodeoxycholate may substitute for Triton X-100 at 0.5% Shewanella algae
3.5.1.69 Triton X-100 required, 0.1% Shewanella algae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.69
-
 Shewanella algae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.69 EDTA 5 mM, strong Shewanella algae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.69 Ca2+ activation Shewanella algae
3.5.1.69 Mg2+ activation Shewanella algae
3.5.1.69 Mn2+ activation Shewanella algae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.69 75000
-
 1 * 109800, deduced from gene sequence, 1 * 75000, SDS-PAGE Shewanella algae
3.5.1.69 109800
-
 1 * 109800, deduced from gene sequence, 1 * 75000, SDS-PAGE Shewanella algae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.69 Shewanella algae Q8RTY6
-
-
3.5.1.69 Shewanella algae G8 Q8RTY6
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.69 proteolytic modification C-terminal processing of enzyme is necessary for full activity Shewanella algae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.69
-
 Shewanella algae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.69 30.4
-
 pH 6.0, 37°C Shewanella algae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.69 asialoganglioside GM1 + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
3.5.1.69 asialoganglioside GM1 + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
3.5.1.69 ganglioside GD1b + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
3.5.1.69 ganglioside GD1b + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
3.5.1.69 ganglioside GM1a + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
3.5.1.69 ganglioside GM1a + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
3.5.1.69 ganglioside GM3 + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
3.5.1.69 ganglioside GM3 + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
3.5.1.69 ganglioside GT1b + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
3.5.1.69 ganglioside GT1b + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.69 monomer 1 * 109800, deduced from gene sequence, 1 * 75000, SDS-PAGE Shewanella algae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.69 5.5 6.5
-
 Shewanella algae