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Literature summary extracted from

  • Shi, J.; Boyd, A.E.; Radic, Z.; Taylor, P.
    Reversibly bound and covalently attached ligands induce conformational changes in the omega loop, Cys69-Cys96, of mouse acetylcholinesterase (2001), J. Biol. Chem., 276, 42196-42204.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7 truncated form terminated at position 548, expressed in HEK-293 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 A262C kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme Mus musculus
3.1.1.7 E81C kinetics similar to wild-type enzyme Mus musculus
3.1.1.7 E84C 4fold increased Km, 2fold increase in Kd for edrophonium binding, 18fold increase for 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide binding Mus musculus
3.1.1.7 H287C kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme Mus musculus
3.1.1.7 L76C kinetics similar to wild-type enzyme Mus musculus
3.1.1.7 Y124C kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide
-
 Mus musculus
3.1.1.7 edrophonium
-
 Mus musculus
3.1.1.7 fasciculin
-
 Mus musculus
3.1.1.7 O-O-dimethyl-O-(2,2-dichlorovinyl)phosphate phosphorylates the active site serine Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.054
-
 acetylthiocholine iodide wild-type enzyme Mus musculus
3.1.1.7 0.057
-
 acetylthiocholine iodide E81C mutant Mus musculus
3.1.1.7 0.058
-
 acetylthiocholine iodide H287C mutant Mus musculus
3.1.1.7 0.059
-
 acetylthiocholine iodide A262C mutant Mus musculus
3.1.1.7 0.065
-
 acetylthiocholine iodide Y124C mutant Mus musculus
3.1.1.7 0.097
-
 acetylthiocholine iodide L76C mutant Mus musculus
3.1.1.7 0.19
-
 acetylthiocholine iodide E84C mutant Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylthiocholine iodide + H2O
-
 Mus musculus thiocholine iodide + acetate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.7 2330
-
 acetylthiocholine iodide Y124C mutant Mus musculus
3.1.1.7 2333
-
 acetylthiocholine iodide Y124C mutant Mus musculus
3.1.1.7 2667
-
 acetylthiocholine iodide wild-type enzyme Mus musculus
3.1.1.7 2667
-
 acetylthiocholine iodide A262C mutant Mus musculus
3.1.1.7 2667
-
 acetylthiocholine iodide E81C mutant Mus musculus
3.1.1.7 2670
-
 acetylthiocholine iodide wild-type enzyme Mus musculus
3.1.1.7 2670
-
 acetylthiocholine iodide A262C mutant Mus musculus
3.1.1.7 2670
-
 acetylthiocholine iodide E81C mutant Mus musculus
3.1.1.7 3000
-
 acetylthiocholine iodide H287C mutant Mus musculus
3.1.1.7 3000
-
 acetylthiocholine iodide L76C mutant Mus musculus
3.1.1.7 3167
-
 acetylthiocholine iodide E84C mutant Mus musculus
3.1.1.7 3170
-
 acetylthiocholine iodide E84C mutant Mus musculus