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Literature summary extracted from

  • Siebers, B.; Brinkmann, H.; Dorr, C.; Tjaden, B.; Lilie, H.; van der Oost, J.; Verhees, C.H.
    Archaeal fructose-1,6-bisphosphate aldolases constitute a new family of archaeal type class I aldolase (2001), J. Biol. Chem., 276, 28710-28718.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13 expressed in Escherichia coli Thermoproteus tenax
4.1.2.13 expressed in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.0036
-
 fructose 1,6-bisphosphate 50°C, pH 7 Thermoproteus tenax
4.1.2.13 0.009
-
 fructose 1,6-bisphosphate 50°C, pH 7 Pyrococcus furiosus
4.1.2.13 0.71
-
 fructose 1-phosphate 50°C, pH 7 Thermoproteus tenax
4.1.2.13 4.48
-
 fructose 1-phosphate 50°C, pH 7 Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 28700
-
 8 * 28700, SDS-PAGE Thermoproteus tenax
4.1.2.13 31100
-
 8 * 31100, SDS-PAGE Pyrococcus furiosus
4.1.2.13 241000
-
 gel filtration Thermoproteus tenax
4.1.2.13 272000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Pyrococcus furiosus P58314
-
-
4.1.2.13 Thermoproteus tenax P58315
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13 recombinant protein Thermoproteus tenax
4.1.2.13 recombinant protein Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate preferred substrate, reaction intermediate is Schiff base Thermoproteus tenax glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate preferred substrate, reaction intermediate is Schiff base Pyrococcus furiosus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-Fructose 1-phosphate
-
 Thermoproteus tenax Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.13 D-Fructose 1-phosphate
-
 Pyrococcus furiosus Glycerone phosphate + D-glyceraldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.13 octamer 8 * 28700, SDS-PAGE Thermoproteus tenax
4.1.2.13 octamer 8 * 31100, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.13 50
-
 assay at Thermoproteus tenax
4.1.2.13 50
-
 assay at Pyrococcus furiosus