EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.23 | wild-type and mutant ODCase, expression in Escherichia coli SS6130 | Saccharomyces cerevisiae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.23 | in complex with 6-hydroxyuridine 5-phosphate | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.23 | D91A | inactive mutant, reduced activity by 5 orders of magnitude, no substrate binding | Saccharomyces cerevisiae |
4.1.1.23 | D96A | inactive mutant, reduced kcat-value by more than 5 orders of magnitude, 11fold decrease in the affinity for the substrate in the ground state | Saccharomyces cerevisiae |
4.1.1.23 | K59A | 130fold reduced kcat-value, 900fold increased Km-value | Saccharomyces cerevisiae |
4.1.1.23 | K93A | inactive mutant, reduced activity by 5 orders of magnitude, no substrate binding | Saccharomyces cerevisiae |
4.1.1.23 | Q215A | same activity as wild-type enzyme | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.23 | 6-azauridine 5'-phosphate | - |
Saccharomyces cerevisiae | |
4.1.1.23 | 6-hydroxyuridine 5'-phosphate | - |
Saccharomyces cerevisiae | |
4.1.1.23 | UMP | - |
Saccharomyces cerevisiae | |
4.1.1.23 | xanthosine 5'-phosphate | - |
Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.23 | additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
4.1.1.23 | 0.0007 | - |
orotidine 5'-phosphate | wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.008 | - |
2'-deoxyorotidine 5'-phosphate | wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.025 | - |
orotidine 5'-phosphate | Q215A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.64 | - |
orotidine 5'-phosphate | K59A mutant | Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.23 | additional information | no metal cofactor requirement | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.23 | Orotidine 5'-phosphate | Saccharomyces cerevisiae | - |
UMP + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.23 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.23 | recombinant wild-type and mutant enzyme | Saccharomyces cerevisiae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.1.1.23 | orotidine 5'-phosphate = UMP + CO2 | mechanism | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.23 | 0.00018 | - |
below, K93A mutant | Saccharomyces cerevisiae |
4.1.1.23 | 0.00047 | - |
below, D96A muatnt | Saccharomyces cerevisiae |
4.1.1.23 | 0.00066 | - |
below, D91A mutant | Saccharomyces cerevisiae |
4.1.1.23 | 0.19 | - |
K59A mutant | Saccharomyces cerevisiae |
4.1.1.23 | 84 | - |
Q215A mutant | Saccharomyces cerevisiae |
4.1.1.23 | 90 | - |
wild-type enzyme | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.23 | 2'-deoxyorotidine 5'-phosphate | - |
Saccharomyces cerevisiae | 2'-deoxyuridine 5'-phosphate + CO2 | - |
? | |
4.1.1.23 | Orotidine 5'-phosphate | - |
Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
4.1.1.23 | Orotidine 5'-phosphate | the integrity of the network of the charged residues within the active site, Lys-59, Asp-91, Lys-93 and Asp-96, is essential for transition state stabilization, altered substrate is very tightly bound in the transition state, mechanism | Saccharomyces cerevisiae | UMP + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.23 | dimer | - |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.23 | 0.00023 | - |
orotidine 5'-phosphate | below, D96A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.34 | - |
orotidine 5'-phosphate | K59A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 41 | - |
orotidine 5'-phosphate | Q215A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 44 | - |
orotidine 5'-phosphate | wild-type enzyme | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.23 | additional information | no cofactor requirement | Saccharomyces cerevisiae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.23 | 0.000000008 | - |
6-hydroxyuridine 5'-phosphate | wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.000029 | - |
6-azauridine 5'-phosphate | Q215A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.000036 | - |
6-azauridine 5'-phosphate | wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.000094 | - |
6-hydroxyuridine 5'-phosphate | K59A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.00041 | - |
xanthosine 5'-phosphate | wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.00046 | - |
xanthosine 5'-phosphate | Q215A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.039 | - |
6-azauridine 5'-phosphate | K59A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.17 | - |
UMP | Q215A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.2 | - |
UMP | wild-type enzyme | Saccharomyces cerevisiae | |
4.1.1.23 | 0.33 | - |
UMP | K59A mutant | Saccharomyces cerevisiae | |
4.1.1.23 | 0.73 | - |
xanthosine 5'-phosphate | K59A mutant | Saccharomyces cerevisiae |