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Literature summary extracted from

  • Miller, B.G.; Snider, M.J.; Wolfenden, R.; Short, S.A.
    Dissecting a charged network at the active site of orotidine-5'-phosphate decarboxylase (2001), J. Biol. Chem., 276, 15174-15176.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.23 wild-type and mutant ODCase, expression in Escherichia coli SS6130 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.23 in complex with 6-hydroxyuridine 5’-phosphate Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.23 D91A inactive mutant, reduced activity by 5 orders of magnitude, no substrate binding Saccharomyces cerevisiae
4.1.1.23 D96A inactive mutant, reduced kcat-value by more than 5 orders of magnitude, 11fold decrease in the affinity for the substrate in the ground state Saccharomyces cerevisiae
4.1.1.23 K59A 130fold reduced kcat-value, 900fold increased Km-value Saccharomyces cerevisiae
4.1.1.23 K93A inactive mutant, reduced activity by 5 orders of magnitude, no substrate binding Saccharomyces cerevisiae
4.1.1.23 Q215A same activity as wild-type enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.23 6-azauridine 5'-phosphate
-
Saccharomyces cerevisiae
4.1.1.23 6-hydroxyuridine 5'-phosphate
-
Saccharomyces cerevisiae
4.1.1.23 UMP
-
Saccharomyces cerevisiae
4.1.1.23 xanthosine 5'-phosphate
-
Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.23 additional information
-
additional information
-
Saccharomyces cerevisiae
4.1.1.23 0.0007
-
orotidine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.008
-
2'-deoxyorotidine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.025
-
orotidine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
4.1.1.23 0.64
-
orotidine 5'-phosphate K59A mutant Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.23 additional information no metal cofactor requirement Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.23 Orotidine 5'-phosphate Saccharomyces cerevisiae
-
UMP + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.23 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.23 recombinant wild-type and mutant enzyme Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.23 orotidine 5'-phosphate = UMP + CO2 mechanism Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.23 0.00018
-
below, K93A mutant Saccharomyces cerevisiae
4.1.1.23 0.00047
-
below, D96A muatnt Saccharomyces cerevisiae
4.1.1.23 0.00066
-
below, D91A mutant Saccharomyces cerevisiae
4.1.1.23 0.19
-
K59A mutant Saccharomyces cerevisiae
4.1.1.23 84
-
Q215A mutant Saccharomyces cerevisiae
4.1.1.23 90
-
wild-type enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.23 2'-deoxyorotidine 5'-phosphate
-
Saccharomyces cerevisiae 2'-deoxyuridine 5'-phosphate + CO2
-
?
4.1.1.23 Orotidine 5'-phosphate
-
Saccharomyces cerevisiae UMP + CO2
-
?
4.1.1.23 Orotidine 5'-phosphate the integrity of the network of the charged residues within the active site, Lys-59, Asp-91, Lys-93 and Asp-96, is essential for transition state stabilization, altered substrate is very tightly bound in the transition state, mechanism Saccharomyces cerevisiae UMP + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.23 dimer
-
Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.23 0.00023
-
orotidine 5'-phosphate below, D96A mutant Saccharomyces cerevisiae
4.1.1.23 0.34
-
orotidine 5'-phosphate K59A mutant Saccharomyces cerevisiae
4.1.1.23 41
-
orotidine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
4.1.1.23 44
-
orotidine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.23 additional information no cofactor requirement Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.23 0.000000008
-
6-hydroxyuridine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.000029
-
6-azauridine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
4.1.1.23 0.000036
-
6-azauridine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.000094
-
6-hydroxyuridine 5'-phosphate K59A mutant Saccharomyces cerevisiae
4.1.1.23 0.00041
-
xanthosine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.00046
-
xanthosine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
4.1.1.23 0.039
-
6-azauridine 5'-phosphate K59A mutant Saccharomyces cerevisiae
4.1.1.23 0.17
-
UMP Q215A mutant Saccharomyces cerevisiae
4.1.1.23 0.2
-
UMP wild-type enzyme Saccharomyces cerevisiae
4.1.1.23 0.33
-
UMP K59A mutant Saccharomyces cerevisiae
4.1.1.23 0.73
-
xanthosine 5'-phosphate K59A mutant Saccharomyces cerevisiae