EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | phosphatidylcholine | 0.2 mM, 2fold activation | Bos taurus | |
3.1.4.12 | phosphatidylethanolamine | 0.2 mM, 2.9fold activation | Bos taurus | |
3.1.4.12 | phosphatidylserine | 0.2 mM, 3.8fold activation | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Ca2+ | 0.1 mM, 60% inhibition of Mg2+-activated N-SMase | Bos taurus | |
3.1.4.12 | EDTA | 10 mM, 95% inhibition | Bos taurus | |
3.1.4.12 | glutathione | 0.2 mM, almost complete inhibition | Bos taurus | |
3.1.4.12 | scyphostatin | 0.02 mM, complete inhibition | Bos taurus | |
3.1.4.12 | Zn2+ | almost complete inhibition of Mg2+-activated N-SMase | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.12 | 0.04 | - |
sphingomyelin | 37°C, pH 7.4 | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.12 | plasma membrane | associated | Bos taurus | 5886 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Mg2+ | required for activity, approx. 3fold activation at 0.1-0.5 mM | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 97000 | - |
x * 97000, 2D-electrophoresis | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | Bos taurus | - |
N-acylsphingosine + choline phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.12 | NP-40, CM-Sepharose, concanavalin A-Sepharose, Methyl-macroprep, isoelectric focusing | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.12 | brain | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 0.23 | - |
- |
Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | - |
Bos taurus | N-acylsphingosine + choline phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.12 | ? | x * 97000, 2D-electrophoresis | Bos taurus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | N-SMase | - |
Bos taurus |
3.1.4.12 | neutral sphingomyelinase | - |
Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 56 | - |
95% loss of activity after 5 min | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 7 | 8 | - |
Bos taurus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 6 | 9 | approx. 30% of maximal activity at pH 6.5, approx. 70% of maximal activity at pH 9.0 | Bos taurus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.4.12 | Bos taurus | isoelectric focusing | - |
5.3 |