EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | arachidonic acid | approx. 3fold activation at 0.5 mM | Mus musculus | |
3.1.4.12 | arachidonic acid | approx. 3fold activation at 0.5 mM | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.12 | expression in Escherichia coli and HEK293 kidney cells | Mus musculus |
3.1.4.12 | expression in Escherichia coli and HEK293 kidney cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.12 | E49Q | complete loss of hnSMase activity | Homo sapiens |
3.1.4.12 | H272N | complete loss of hnSMase activity | Homo sapiens |
3.1.4.12 | N180H | complete loss of hnSMase activity | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Mn2+ | above 0.5 mM | Homo sapiens | |
3.1.4.12 | Mn2+ | above 0.5 mM | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.12 | smooth endoplasmic reticulum | - |
Mus musculus | 5790 | - |
3.1.4.12 | smooth endoplasmic reticulum | - |
Homo sapiens | 5790 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Mg2+ | required for activity, maximal activation at 2-5 mM | Mus musculus | |
3.1.4.12 | Mg2+ | required for activity, maximal activation at 2-5 mM | Homo sapiens | |
3.1.4.12 | Mn2+ | can replace Mg2+, maximal activity at 0.5 mM, inhibition above | Mus musculus | |
3.1.4.12 | Mn2+ | can replace Mg2+, maximal activity at 0.5 mM, inhibition above | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 42000 | - |
x * 42000, SDS-PAGE | Mus musculus |
3.1.4.12 | 42000 | - |
x * 42000, SDS-PAGE | Homo sapiens |
3.1.4.12 | 47500 | - |
x * 47500, deduced from nucleotide sequence | Mus musculus |
3.1.4.12 | 47600 | - |
x * 47600, deduced from nucleotide sequence | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | Mus musculus | - |
N-acylsphingosine + choline phosphate | - |
? | |
3.1.4.12 | sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Homo sapiens | - |
- |
- |
3.1.4.12 | Mus musculus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.4.12 | no glycoprotein | - |
Mus musculus |
3.1.4.12 | no glycoprotein | - |
Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.12 | affinity purification of recombinant hnSMase | Homo sapiens |
3.1.4.12 | affinity purification of recombinant mnSMase | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.12 | brain | - |
Mus musculus | - |
3.1.4.12 | brain | - |
Homo sapiens | - |
3.1.4.12 | heart | - |
Mus musculus | - |
3.1.4.12 | heart | - |
Homo sapiens | - |
3.1.4.12 | kidney | predominant tissue | Mus musculus | - |
3.1.4.12 | kidney | predominant tissue | Homo sapiens | - |
3.1.4.12 | liver | - |
Mus musculus | - |
3.1.4.12 | liver | - |
Homo sapiens | - |
3.1.4.12 | lung | - |
Mus musculus | - |
3.1.4.12 | lung | - |
Homo sapiens | - |
3.1.4.12 | muscle | - |
Mus musculus | - |
3.1.4.12 | muscle | - |
Homo sapiens | - |
3.1.4.12 | spleen | - |
Mus musculus | - |
3.1.4.12 | spleen | - |
Homo sapiens | - |
3.1.4.12 | testis | - |
Mus musculus | - |
3.1.4.12 | testis | - |
Homo sapiens | - |
3.1.4.12 | thymus | - |
Mus musculus | - |
3.1.4.12 | thymus | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | - |
Mus musculus | N-acylsphingosine + choline phosphate | - |
? | |
3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.12 | ? | x * 42000, SDS-PAGE | Mus musculus |
3.1.4.12 | ? | x * 42000, SDS-PAGE | Homo sapiens |
3.1.4.12 | ? | x * 47500, deduced from nucleotide sequence | Mus musculus |
3.1.4.12 | ? | x * 47600, deduced from nucleotide sequence | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | hnSMase | - |
Homo sapiens |
3.1.4.12 | magnesium-dependent neutral sphingomyelinase | - |
Mus musculus |
3.1.4.12 | magnesium-dependent neutral sphingomyelinase | - |
Homo sapiens |
3.1.4.12 | mnSMase | - |
Mus musculus |