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Literature summary extracted from

  • Xian, M.; Chen, X.; Liu, Z.; Wang, K.; Wang, P.G.
    Inhibition of papain by S-nitrosothiols. Formation of mixed disulfides (2000), J. Biol. Chem., 275, 20467-20473.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 acetyl-Phe-Gly-S-nitrosopenicillamine inactivation of papain by S-nitrosothiols is due to a direct attack of the highly reactive thiolate Cys25 in the enzyme active site on the sulfur of S-nitrosothiols to form a mixed disulfide between the inactivator and papain Carica papaya
3.4.22.2 glucose-2S-nitroso-N-acetyl-penicillamine inactivation of papain by S-nitrosothiols is due to a direct attack of the highly reactive thiolate Cys25 in the enzyme active site on the sulfur of S-nitrosothiols to form a mixed disulfide between the inactivator and papain Carica papaya
3.4.22.2 S-nitroso-N-acetyl-DL-penicillamine inactivation of papain by S-nitrosothiols is due to a direct attack of the highly reactive thiolate Cys25 in the enzyme active site on the sulfur of S-nitrosothiols to form a mixed disulfide between the inactivator and papain Carica papaya
3.4.22.2 S-nitrosocaptopril inactivation of papain by S-nitrosothiols is due to a direct attack of the highly reactive thiolate Cys25 in the enzyme active site on the sulfur of S-nitrosothiols to form a mixed disulfide between the inactivator and papain Carica papaya
3.4.22.2 S-nitrosoglutathione inactivation of papain by S-nitrosothiols is due to a direct attack of the highly reactive thiolate Cys25 in the enzyme active site on the sulfur of S-nitrosothiols to form a mixed disulfide between the inactivator and papain Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 N-benzyloxycarbonyl-Gly-p-nitroanilide + H2O
-
 Carica papaya ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.2 0.096
-
 acetyl-Phe-Gly-S-nitrosopenicillamine pH 6.2, 25°C Carica papaya
3.4.22.2 0.305
-
 S-nitrosoglutathione pH 6.2, 25°C Carica papaya
3.4.22.2 0.356
-
 S-nitroso-N-acetyl-DL-penicillamine pH 6.2, 25°C Carica papaya
3.4.22.2 0.449
-
 S-nitrosocaptopril pH 6.2, 25°C Carica papaya
3.4.22.2 0.618
-
 D-glucose-2S-nitroso-N-acetyl-penicillamine pH 6.2, 25°C Carica papaya