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Literature summary extracted from

  • Kester, H.C.M.; Magaud, D.; Roy, C.; Anker, D.; Doutheau, A.; Shevchik, V.; Hugouvieux-Cotte-Pattat, N.; Benen, J.A.E.; Visser, J.
    Performance of selected microbial pectinases on synthetic monomethyl-esterified di- and trigalacturonates (1999), J. Biol. Chem., 274, 37053-37059.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.6 Dickeya chrysanthemi
-
-
-
4.2.2.9 Aspergillus tubingensis
-
-
-
4.2.2.9 Dickeya chrysanthemi
-
 2 different enzyme types: exopolygalacturonate lyase X, i.e. Pel X, and exopectate lyase W, i.e. PelW
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.6 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate = 2 5-dehydro-4-deoxy-D-glucuronate also catalyses eliminative removal of unsaturated terminal residues from oligosaccharides of D-galacturonate Dickeya chrysanthemi
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.6 additional information
-
 substrate specificity Dickeya chrysanthemi
4.2.2.9 additional information
-
 substrate specificity Dickeya chrysanthemi
4.2.2.9 additional information
-
 substrate specificity Aspergillus tubingensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.6 1-methyl-di-D-galacturonate alpha-(1-4)-linked Dickeya chrysanthemi ?
-
 ?
4.2.2.6 1-methyl-tri-D-galacturonate alpha-(1-4)-linked Dickeya chrysanthemi ?
-
 ?
4.2.2.6 3-methyl-tri-D-galacturonate best substrate, alpha-(1-4)-linked Dickeya chrysanthemi ?
-
 ?
4.2.2.6 DELTA4,5-unsaturated di-D-galacturonate alpha-(1-4)-linked Dickeya chrysanthemi ?
-
 ?
4.2.2.6 DELTA4,5-unsaturated tri-D-galacturonate alpha-(1-4)-linked Dickeya chrysanthemi ?
-
 ?
4.2.2.6 di-D-galacturonate
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.6 additional information no activity with 2-methyl-di-G-galacturonate and 2-methyl-tri-G-galacturonate Dickeya chrysanthemi ?
-
 ?
4.2.2.6 tri-D-galacturonate
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.9 1-methyl-di-D-galacturonate alpha-(1-4)-linked, 36% of the activity with di-D-galacturonate Aspergillus tubingensis ?
-
 ?
4.2.2.9 1-methyl-tri-D-galacturonate alpha-(1-4)-linked Dickeya chrysanthemi ?
-
 ?
4.2.2.9 1-methyl-tri-D-galacturonate best substrate, alpha-(1-4)-linked Aspergillus tubingensis ?
-
 ?
4.2.2.9 2-methyl-di-D-galacturonate alpha-(1-4)-linked, 35% of the activity with di-D-galacturonate Aspergillus tubingensis ?
-
 ?
4.2.2.9 2-methyl-tri-D-galacturonate alpha-(1-4)-linked, 19% of the activity with tri-D-galacturonate or 1-methyl-tri-D-galacturonate Aspergillus tubingensis ?
-
 ?
4.2.2.9 3-methyl-tri-D-galacturonate alpha-(1-4)-linked, 18% of the activity with tri-D-galacturonate or 1-methyl-tri-D-galacturonate Aspergillus tubingensis ?
-
 ?
4.2.2.9 3-methyl-tri-D-galacturonate poor substrate only for PelW, not for PelX Dickeya chrysanthemi ?
-
 ?
4.2.2.9 DELTA4,5-unsaturated tri-D-galacturonate alpha-(1-4)-linked, low activity with PelX Dickeya chrysanthemi ?
-
 ?
4.2.2.9 di-D-galacturonate alpha-(1-4)-linked Aspergillus tubingensis ?
-
 ?
4.2.2.9 additional information substrate specificity, overview, no activity for both enzyme types with monomethyl-esterified digalacturonate substrates and with 2-methyl-tri-G-galacturonate Dickeya chrysanthemi ?
-
 ?
4.2.2.9 tri-D-galacturonate alpha-(1-4)-linked Aspergillus tubingensis ?
-
 ?
4.2.2.9 tri-D-galacturonate alpha-(1-4)-linked, best substrate for both enzyme types Dickeya chrysanthemi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.6 Ogl
-
 Dickeya chrysanthemi
4.2.2.6 oligogalacturonan lyase
-
 Dickeya chrysanthemi
4.2.2.9 exopectate lyase W
-
 Dickeya chrysanthemi
4.2.2.9 exopolygalacturonase
-
 Aspergillus tubingensis
4.2.2.9 exopolygalacturonate lyase X
-
 Dickeya chrysanthemi
4.2.2.9 PelW
-
 Dickeya chrysanthemi
4.2.2.9 PelX
-
 Dickeya chrysanthemi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.6 37
-
 assay at Dickeya chrysanthemi
4.2.2.9 37
-
 assay at Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.6 8
-
 assay at Dickeya chrysanthemi
4.2.2.9 8
-
 assay at Dickeya chrysanthemi