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Literature summary extracted from

  • Thoden, J.B.; Raushel, F.M.; Wesenberg, G.; Holden, H.M.
    The binding of inosine monophosphate to Escherichia coli carbamoyl phosphate synthetase (1999), J. Biol. Chem., 274, 22502-22507.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 L-ornithine
-
 Escherichia coli
6.3.5.5 potassium
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.5.5 in presence of both IMP and ornithine, structural analysis of the nucleotide monophosphate allosteric binding site for enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 additional information IMP binds to enzyme but the actual effect of this ligand is dependent upon temperature and assay conditions Escherichia coli
6.3.5.5 UMP
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3- + H+ + H2O Escherichia coli the product carbamoyl phosphate is utilized in the pyrimidine and arginine biosynthetic pathways 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3- + H+ + H2O the product carbamoyl phosphate is utilized in the pyrimidine and arginine biosynthetic pathways Escherichia coli 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 heterodimer alpha,beta, that readly coverts to an tetrameric species: 4 * alpha, beta in presence of positive allosteric effectors Escherichia coli