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Literature summary extracted from

  • Chen, Y.R.; Larimer, F.W.; Serpersu, E.H.; Hartman, F.C.
    Identification of a catalytic aspartyl residue of D-ribulose 5-phosphate 3-epimerase by site-directed mutagenesis (1999), J. Biol. Chem., 274, 2132-2136.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.1 D186A KM-value for D-ribulose 5-phosphate is increased 10fold, turnover-number is decreased to less than 0.1% of the wild-type value Spinacia oleracea
5.1.3.1 D186E KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value Spinacia oleracea
5.1.3.1 D186N KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.1 0.22
-
 D-ribulose 5-phosphate wild-type enzyme Spinacia oleracea
5.1.3.1 0.28
-
 D-ribulose 5-phosphate mutant enzyme D186N Spinacia oleracea
5.1.3.1 0.3
-
 D-ribulose 5-phosphate mutant enzyme D186E Spinacia oleracea
5.1.3.1 2.1
-
 D-ribulose 5-phosphate mutant enzyme D186A Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.1 Spinacia oleracea Q43157
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.1 mutant enzymes D186A, D186N, D186E Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.1 D-Ribulose 5-phosphate
-
 Spinacia oleracea D-Xylulose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.3.1 Ru5P epimerase
-
 Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.1 0.00033
-
 D-ribulose 5-phosphate mutant enzyme D186A Spinacia oleracea
5.1.3.1 0.13
-
 D-ribulose 5-phosphate mutant enzyme D186N Spinacia oleracea
5.1.3.1 1.1
-
 D-ribulose 5-phosphate mutant enzyme D186E Spinacia oleracea
5.1.3.1 7100
-
 D-ribulose 5-phosphate wild-type enzyme Spinacia oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.3.1 Spinacia oleracea wild-type enzyme and mutant enzyme D186E, denaturing isoelectric focusing, pH gradient 5-8 6.8 6.3
5.1.3.1 Spinacia oleracea mutant enzymes D186A and D186N, denaturing isoelectric focusing, pH gradient 5-8
-
 6.6