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Literature summary extracted from

  • Knaeuper, V.; Cowell, S.; Smith, B.; Lopez-Otin, C.; O'Shea, M.; Morris, H.; Zardi, L.; Murphy, G.
    The role of the C-terminal domain of human collagenase-3 (MMP-13) in the activation of procollagenase-3, substrate specificity, and tissue inhibitor of metalloproteinase interaction (1997), J. Biol. Chem., 272, 7608-7616.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.B4 amino phenylmercuric acetate activation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B4 activation by aminophenylmercuric acetate, expression of procollagenase 3 and a C-terminal truncated form of procollagenase 3 comprising amino acid residues 249-451 in mouse myeloma cells, secretion to the cell culture medium Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B4 tissue matrix metalloproteinase inhibitor fragments of TIMP-1
-
 Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor fragments of TIMP-2
-
 Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor hybrid N.TIMP-1/C.TIMP-2 complex formation is not affected by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor TIMP-1 complex formation is promoted by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor TIMP-2 complex formation is not affected by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor TIMP-3 complex formation is promoted by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B4 24000
-
 x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE Homo sapiens
3.4.24.B4 35000
-
 x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE Homo sapiens
3.4.24.B4 50000
-
 x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE Homo sapiens
3.4.24.B4 60000
-
 x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B4 protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B4 proteolytic modification autoproteolysis resulting in two N-glycosylated 29 kDa fragments and one 27 kDa fragment, processing of enzyme proform via a short-living intermediate form to the active form with Tyr85 at its N-terminus Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B4 recombinant enzyme and truncated mutant from myeloma cell culture medium Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.B4 additional information
-
 activity of enzyme and enzyme fragment with synthetic peptide substrates Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B4 (7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly-norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly + norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2
-
 ?
3.4.24.B4 (7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly-Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly + Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2
-
 ?
3.4.24.B4 Collagen type I + H2O wild-type enzyme, substrate binding is mediated by the C-terminal domain, which is essential for the triple helicase activity Homo sapiens ?
-
 ?
3.4.24.B4 collagen type I beta1,2 chain + H2O wild-type enzyme and enzyme fragment 249-451 Homo sapiens collagen type I alpha1,2 chain
-
 ?
3.4.24.B4 collagen type II + H2O wild-type enzyme, soluble substrate Homo sapiens ?
-
 ?
3.4.24.B4 collagen type IV + H2O substrate alpha2-chain and alpha1-chain, wild-type enzyme and enzyme fragment 249-451 Homo sapiens ?
-
 ?
3.4.24.B4 collagen type IV + H2O wild-type enzyme and enzyme fragment 249-451 Homo sapiens ?
-
 ?
3.4.24.B4 collagen type IX + H2O wild-type enzyme and enzyme fragment 249-451 Homo sapiens ?
-
 ?
3.4.24.B4 collagen type X + H2O wild-type enzyme and enzyme fragment 249-451 Homo sapiens 48 kDa fragment
-
 ?
3.4.24.B4 collagen type XIV + H2O alpha3-chain, wild-type enzyme and enzyme fragment 249-451 Homo sapiens ?
-
 ?
3.4.24.B4 fibronectin + H2O native and recombinant substrate, whole substrate molecule and fragments, wild-type enzyme and enzyme fragment 249-451 Homo sapiens fragments of MW 100 kDa, 43 kDa, 35 kDa, and 29 kDa from intact plasma fibronectin ?
3.4.24.B4 large tenascin C isoform + H2O wild-type enzyme and enzyme fragment 249-451 Homo sapiens ?
-
 ?
3.4.24.B4 additional information no activity with the minor cartilage collagen type XI Homo sapiens ?
-
 ?
3.4.24.B4 protein + H2O
-
 Homo sapiens peptides
-
 ?
3.4.24.B4 protein + H2O substrate specificity, overview Homo sapiens peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B4 ? x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B4 M10.013 Merops-ID Homo sapiens
3.4.24.B4 matrix metalloproteinase-13
-
 Homo sapiens
3.4.24.B4 MMP-13
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.B4 additional information
-
 additional information tissue matrix metalloproteinase inhibitors and fragments Homo sapiens