Literature summary extracted from

Sun, Z.; Jiang, Y.; Ma, Z.; Wu, H.; Liu, B.F.; Xue, Y.; Tang, W.; Chen, Y.; Li, C.; Zhu, D.; Gurewich, V.; Liu, J.N.; Zhong, M.; Xu, Y.
Identification of a flexible loop region (297-313) of urokinase-type plasminogen activator, which helps determine its catalytic activity (1997), J. Biol. Chem., 272, 23818-23823.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.21.73	cDNA cloning	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.21.73	E301A	site-directed mutagenesis	Homo sapiens
3.4.21.73	E301D	site-directed mutagenesis	Homo sapiens
3.4.21.73	E301H	site-directed mutagenesis	Homo sapiens
3.4.21.73	K300A	site-directed mutagenesis	Homo sapiens
3.4.21.73	K300H	site-directed mutagenesis	Homo sapiens
3.4.21.73	K300W	site-directed mutagenesis	Homo sapiens
3.4.21.73	K313A	site-directed mutagenesis	Homo sapiens
3.4.21.73	S303E	site-directed mutagenesis	Homo sapiens
3.4.21.73	Y306G	site-directed mutagenesis	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.21.73	0.051	-	S2444	pH 7.4, 25°C, wild-type and mutant E301A, single-chain activator	Homo sapiens
3.4.21.73	0.052	-	S2444	pH 7.4, 25°C, mutant K300H, single-chain activator	Homo sapiens
3.4.21.73	0.053	-	S2444	pH 7.4, 25°C, mutant S303E, single-chain activator	Homo sapiens
3.4.21.73	0.054	-	S2444	pH 7.4, 25°C, mutant K300A and K313A, single-chain activator	Homo sapiens
3.4.21.73	0.055	-	S2444	pH 7.4, 25°C, mutant E301H, single-chain activator	Homo sapiens
3.4.21.73	0.058	-	S2444	pH 7.4, 25°C, mutant Y306G, single-chain activator	Homo sapiens
3.4.21.73	0.059	-	S2444	pH 7.4, 25°C, mutant K300W, single-chain activator	Homo sapiens
3.4.21.73	0.06	-	S2444	pH 7.4, 25°C, mutant E301D, single-chain activator	Homo sapiens
3.4.21.73	0.074	-	S2444	pH 7.4, 25°C, mutant K300W, two-chain activator	Homo sapiens
3.4.21.73	0.075	-	S2444	pH 7.4, 25°C, mutant K300H, two-chain activator	Homo sapiens
3.4.21.73	0.077	-	S2444	pH 7.4, 25°C, mutant K300A and S303E, two-chain activator	Homo sapiens
3.4.21.73	0.078	-	S2444	pH 7.4, 25°C, wild-type, two-chain activator	Homo sapiens
3.4.21.73	0.08	-	S2444	pH 7.4, 25°C, mutant K313A, two-chain activator	Homo sapiens
3.4.21.73	0.082	-	S2444	pH 7.4, 25°C, mutant E301D, two-chain activator	Homo sapiens
3.4.21.73	0.085	-	S2444	pH 7.4, 25°C, mutant Y306G, two-chain activator	Homo sapiens
3.4.21.73	0.087	-	S2444	pH 7.4, 25°C, mutant E301A, two-chain activator	Homo sapiens
3.4.21.73	0.089	-	S2444	pH 7.4, 25°C, mutant E301H, two-chain activator	Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.4.21.73	plasminogen + H2O	Homo sapiens	principal function is fibrinolysis, u-PA also been implicated in other physiological functions such as embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing	plasmin + ?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.73	Homo sapiens	-	human	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.21.73	-	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.73	additional information	urokinase-type plasminogen activator includes its single-chain zymogen, pro-urokinase and two-chain enzyme urokinase	Homo sapiens	?	-	?
3.4.21.73	plasminogen + H2O	-	Homo sapiens	plasmin + ?	-	?
3.4.21.73	plasminogen + H2O	principal function is fibrinolysis, u-PA also been implicated in other physiological functions such as embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing	Homo sapiens	plasmin + ?	-	?
3.4.21.73	S2444 + H2O	i.e. 5-oxo-L-Pro-Gly-L-Arg-p-nitroanilide	Homo sapiens	pyroGlu-Gly-Arg + 4-nitroaniline	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.21.73	UK	-	Homo sapiens
3.4.21.73	Urokinase-type plasminogen activator	-	Homo sapiens

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4.21.73	0.0077	-	S2444	pH 7.4, 25°C, mutant K300A, single-chain activator	Homo sapiens
3.4.21.73	0.0085	-	S2444	pH 7.4, 25°C, mutant K300W, single-chain activator	Homo sapiens
3.4.21.73	0.027	-	S2444	pH 7.4, 25°C, mutant E301A, single-chain activator	Homo sapiens
3.4.21.73	0.029	-	S2444	pH 7.4, 25°C, mutant Y306G, single-chain activator	Homo sapiens
3.4.21.73	0.047	-	S2444	pH 7.4, 25°C, mutant E301D, single-chain activator	Homo sapiens
3.4.21.73	0.065	-	S2444	pH 7.4, 25°C, mutant K300H, single-chain activator	Homo sapiens
3.4.21.73	0.32	-	S2444	pH 7.4, 25°C, mutant S303E, single-chain activator	Homo sapiens
3.4.21.73	0.32	-	S2444	pH 7.4, 25°C, wild-type, single-chain activator	Homo sapiens
3.4.21.73	0.33	-	S2444	pH 7.4, 25°C, mutant K313A, single-chain activator	Homo sapiens
3.4.21.73	1.5	-	S2444	pH 7.4, 25°C, mutant E301H, single-chain activator	Homo sapiens
3.4.21.73	22	-	S2444	pH 7.4, 25°C, mutant Y306G, two-chain activator	Homo sapiens
3.4.21.73	27	-	S2444	pH 7.4, 25°C, mutant K300W, two-chain activator	Homo sapiens
3.4.21.73	60	-	S2444	pH 7.4, 25°C, mutant K300A, two-chain activator	Homo sapiens
3.4.21.73	83	-	S2444	pH 7.4, 25°C, mutant E301H, two-chain activator	Homo sapiens
3.4.21.73	180	-	S2444	pH 7.4, 25°C, wild-type and mutant S303E, two-chain activator	Homo sapiens
3.4.21.73	190	-	S2444	pH 7.4, 25°C, mutant K313A, two-chain activator	Homo sapiens
3.4.21.73	200	-	S2444	pH 7.4, 25°C, mutant E301A, two-chain activator	Homo sapiens
3.4.21.73	270	-	S2444	pH 7.4, 25°C, mutant E301D, two-chain activator	Homo sapiens
3.4.21.73	350	-	S2444	pH 7.4, 25°C, mutant K300H, two-chain activator	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)