EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.B17 | expression of wild-type and mutants as His-Myc-tagged proteins | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.B17 | E415K | site-directed mutagenesis, mutation of the zinc binding sequence motif, reduced proteolytic activity | Escherichia coli |
3.4.24.B17 | K136N | site-directed mutagenesis, mutation is located in a second ATP binding site, activity is slightly reduced, can complement a deficient mutant strain | Escherichia coli |
3.4.24.B17 | K198N | site-directed mutagenesis, mutation is located in the C-terminal ATP binding site, inactive, no complementation of a deficient mutant strain | Escherichia coli |
3.4.24.B17 | S137N | site-directed mutagenesis, mutation is located in a second ATP binding site, activity is slightly reduced, can complement a deficient mutant strain | Escherichia coli |
3.4.24.B17 | T199A | site-directed mutagenesis, mutation is located in the C-terminal ATP binding site, inactive, no complementation of a deficient mutant strain | Escherichia coli |
3.4.24.B17 | T199N | site-directed mutagenesis, mutation is located in the C-terminal ATP binding site, highly reduced activity, weak complementation of a deficient mutant strain | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.B17 | membrane | integral | Escherichia coli | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B17 | Zn2+ | zinc metalloprotease, essential zinc binding sequence motif HEXXH at the cytoplasmic domain | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B17 | protein + H2O | Escherichia coli | involved in membrane protein assembly as well as degradation of unstable proteins | peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.B17 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.B17 | recombinant His-Myc-tagged wild-type and mutants | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B17 | protein + H2O | - |
Escherichia coli | peptides | - |
? | |
3.4.24.B17 | protein + H2O | involved in membrane protein assembly as well as degradation of unstable proteins | Escherichia coli | peptides | - |
? | |
3.4.24.B17 | protein SecY + H2O | substrate protein is a subunit of protein translocase, failed to assemble with its partner SecE | Escherichia coli | ? | - |
? | |
3.4.24.B17 | subunit a of F0 + H2O | F0 part of the proton ATPase | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.B17 | FtsH | - |
Escherichia coli |
3.4.24.B17 | HlB | - |
Escherichia coli |
3.4.24.B17 | M41.001 | Merops-ID | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.B17 | 42 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.B17 | 7.5 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B17 | ATP | dependent on, 2 ATP binding consensus sequences, 1 is located at the C-terminus and is essential for proteolytic activity | Escherichia coli | |
3.4.24.B17 | CTP | ineffective substitute for ATP, low activity | Escherichia coli | |
3.4.24.B17 | UTP | ineffective substitute for ATP, low activity | Escherichia coli |