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Literature summary extracted from

  • Kim, S.J.; Shin, Y.H.; Kim, K.; Park, E.H.; Sa, J.H.; Lim, C.J.
    Regulation of the gene encoding glutathione synthetase from the fission yeast (2003), J. Biochem. Mol. Biol., 36, 326-331.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 expression in Schizosaccharomyces pombe, results in 1.4fold higher glutathione content and 1.9fold ncreased enzyme activity, regulation by the Atf1-Spc-1-Wis1 signal pathway Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 Cd2+ wild-type cells are not able to grow on cadmium chloride containing substrate, but the recombinant strain is able to survive at 1 mM cadmium chloride concentration, the wild-type can grow on 1 mM Cd2+ in presence of 20 mM GSH Schizosaccharomyces pombe
6.3.2.3 Hg2+ wild-type cells are not able to grow on mercuric chloride containing substrate, but the recombinant strain is able to survive at 0.01 mM mercuric chloride concentration, the wild-type can grow on 0.01 mM Hg2+ in presence of 20 mM GSH Schizosaccharomyces pombe
6.3.2.3 L-buthionine-(S,R)-sulfoximine induction of enzyme expression at 0.01 mM Schizosaccharomyces pombe
6.3.2.3 menadione wild-type cells are not able to grow on menadione containing substrate, but the recombinant strain is able to survive at 0.1 mM menadione, induction of enzyme expression by superoxide generation Schizosaccharomyces pombe
6.3.2.3 additional information no induction of enzyme expression by thioacetamide Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly Schizosaccharomyces pombe gene is regulated by stresses via the Atf1-Spc-1-Wis1 signal pathway ADP + phosphate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Schizosaccharomyces pombe
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 additional information
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly
-
 Schizosaccharomyces pombe ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly gene is regulated by stresses via the Atf1-Spc-1-Wis1 signal pathway Schizosaccharomyces pombe ADP + phosphate + glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.3 Glutathione synthetase
-
 Schizosaccharomyces pombe
6.3.2.3 GS
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.3 37
-
 assay at Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 8.2
-
 assay at Schizosaccharomyces pombe