EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.9 | DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.9 | Ca2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
3.4.11.9 | Co2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
3.4.11.9 | Cu2+ | 56% inhibition at 0.1 mM | Drosophila melanogaster | |
3.4.11.9 | EDTA | 80% inhibition at 0.1 mM | Drosophila melanogaster | |
3.4.11.9 | Mg2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
3.4.11.9 | Mn2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin | Drosophila melanogaster | |
3.4.11.9 | Ni2+ | complete inhibition at 0.001 mM | Drosophila melanogaster | |
3.4.11.9 | Zn2+ | complete inhibition at 0.001 mM | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.9 | cytosol | - |
Drosophila melanogaster | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.9 | Ca2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
3.4.11.9 | Co2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
3.4.11.9 | Mg2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
3.4.11.9 | Mn2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin | Drosophila melanogaster | |
3.4.11.9 | additional information | metalloprotease | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.9 | 71000 | - |
x * 71000, recombinant His-tagged enzyme, SDS-PAGE | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.9 | Drosophila melanogaster | O96794 | cytosolic isoform | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.9 | recombinant His-tagged enzyme from Escherichia coli | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.11.9 | brain | adult fly, high level | Drosophila melanogaster | - |
3.4.11.9 | gut | adult fly | Drosophila melanogaster | - |
3.4.11.9 | additional information | developmentally regulated | Drosophila melanogaster | - |
3.4.11.9 | ovary | adult fly | Drosophila melanogaster | - |
3.4.11.9 | testis | adult fly | Drosophila melanogaster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.9 | bradykinin + H2O | i.e. RPPGFSPFR, hydrolysis of the N-terminal Arg1-Pro2 bond | Drosophila melanogaster | Arg + des-Arg-bradykinin | i.e. PPGFSPFR | ? | |
3.4.11.9 | substance P + H2O | i.e. RPKPQQFFGLM, hydrolysis of the N-terminal Arg1-Pro2 bond | Drosophila melanogaster | Arg + des-Arg-substance P | i.e. PKPQQFFGLM | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.9 | ? | x * 71000, recombinant His-tagged enzyme, SDS-PAGE | Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.9 | aminopeptidase P | - |
Drosophila melanogaster |
3.4.11.9 | DAP-P | - |
Drosophila melanogaster |
3.4.11.9 | More | the enzyme belongs to the peptidase family M24 | Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.9 | 37 | - |
assay at | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.9 | 7.6 | - |
with substrate substance P | Drosophila melanogaster |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.9 | 7.2 | 8 | - |
Drosophila melanogaster |