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Literature summary extracted from

  • Shiraki, K.; Norioka, S.; Li, S.; Sakiyama, F.
    Contribution of an imidazole-indole stack to high catalytic potency of a lysine-specific serine protease, Achromobacter protease I (2002), J. Biochem., 131, 213-218.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.50 H210A the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 7.1% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is identical to the wild-type value Achromobacter lyticus
3.4.21.50 H210F the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 14% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 29.5% of the wild-type value Achromobacter lyticus
3.4.21.50 H210S the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 8.6% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 1.7fold higher than the wild-type value Achromobacter lyticus
3.4.21.50 W169A the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 0.5% of the wild-type value Achromobacter lyticus
3.4.21.50 W169F the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 45% of the wild-type value Achromobacter lyticus
3.4.21.50 W169G the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 0.06% of the wild-type value Achromobacter lyticus
3.4.21.50 W169H the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 9% of the wild-type value Achromobacter lyticus
3.4.21.50 W169L the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 25% of the wild-type value Achromobacter lyticus
3.4.21.50 W169V the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 6% of the wild-type value Achromobacter lyticus
3.4.21.50 W169Y the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 43% of the wild-type value Achromobacter lyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.50 0.0007
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
3.4.21.50 0.0013
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 0.0021
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
3.4.21.50 0.0028
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
3.4.21.50 0.0034
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 0.0036
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 0.0037
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
3.4.21.50 0.004
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 0.0043
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 0.0046
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 0.0058
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 0.006
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
3.4.21.50 0.0061
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 0.0063
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 0.0072
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 0.0073
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 0.0099
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 0.01
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 0.01
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
3.4.21.50 0.012
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
3.4.21.50 0.017
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 0.02
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 0.023
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 0.024
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 0.029
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 0.032
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169G Achromobacter lyticus
3.4.21.50 0.034
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 0.034
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 0.037
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 0.038
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
3.4.21.50 0.042
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
3.4.21.50 0.046
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 0.066
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 0.074
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 0.081
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Achromobacter lyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.50 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.50 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.50 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.50 0.4
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 0.8
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169G Achromobacter lyticus
3.4.21.50 1.1
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 2 8 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
3.4.21.50 2.3
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
3.4.21.50 2.6
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 2.7
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 3 6 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 4.5
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 4.6
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
3.4.21.50 5.2
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 6
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 7
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
3.4.21.50 8.4
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
3.4.21.50 11
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 15
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 18
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
3.4.21.50 20
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
3.4.21.50 22
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 23
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 24
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 31
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 37
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
3.4.21.50 40
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 45
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 46
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
3.4.21.50 48
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 51
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 52
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
3.4.21.50 67
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3.4.21.50 71
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
3.4.21.50 83
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
3.4.21.50 93
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
3.4.21.50 96
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
3.4.21.50 98
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus